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排除万难：一例疑似骨结核病例的分子确诊

Against all odds: molecular confirmation of an implausible case of bone tuberculosis.

作者信息

Loïez Caroline, Willery Eve, Legrand J-Louis, Vincent Véronique, Gutierrez Maria Cristina, Courcol René J, Supply Philip

机构信息

Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional Universitaire, Lille, France.

出版信息

Clin Infect Dis. 2006 Jun 1;42(11):e86-8. doi: 10.1086/503571. Epub 2006 Apr 19.

DOI:10.1086/503571
PMID:16652301
Abstract

We report the use of typing based on a variable number of tandem repeats of genetic elements called "mycobacterial interspersed repetitive units" to clarify a puzzling situation involving a patient with an exceptional case of spondylodiskitis that initially led to the suspicion of a possible event of laboratory cross-contamination with Mycobacterium tuberculosis.

摘要

我们报告了基于一种名为“分枝杆菌散布重复单位”的遗传元件串联重复数目的分型方法的应用，以阐明一个令人困惑的情况，该情况涉及一名患有特殊类型脊椎椎间盘炎的患者，最初怀疑可能存在与结核分枝杆菌的实验室交叉污染事件。

相似文献

1
Against all odds: molecular confirmation of an implausible case of bone tuberculosis.排除万难：一例疑似骨结核病例的分子确诊
Clin Infect Dis. 2006 Jun 1;42(11):e86-8. doi: 10.1086/503571. Epub 2006 Apr 19.
2
Molecular typing of Mycobacterium tuberculosis isolates from a rural area of Kanpur by spoligotyping and mycobacterial interspersed repetitive units (MIRUs) typing.通过间隔寡核苷酸分型（spoligotyping）和分枝杆菌插入重复单元（MIRUs）分型对坎普尔农村地区结核分枝杆菌分离株进行分子分型。
Infect Genet Evol. 2008 Sep;8(5):621-6. doi: 10.1016/j.meegid.2008.05.002. Epub 2008 May 9.
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The use of variable-number tandem-repeat mycobacterial interspersed repetitive unit typing to identify laboratory cross-contamination with Mycobacterium tuberculosis.使用可变数目串联重复序列分枝杆菌插入重复单元分型法来鉴定结核分枝杆菌实验室交叉污染。
Diagn Microbiol Infect Dis. 2005 May;52(1):21-8. doi: 10.1016/j.diagmicrobio.2004.12.002.
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Low positive predictive values and specificities of spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat typing methods for performing population-based molecular epidemiology studies of tuberculosis.在进行基于人群的结核病分子流行病学研究时，间隔寡核苷酸分型（spoligotyping）和分枝杆菌插入重复单位-可变数目串联重复序列分型（mycobacterial interspersed repetitive unit-variable-number tandem repeat typing）方法的阳性预测值和特异性较低。
J Clin Microbiol. 2005 Jun;43(6):3031; author reply 3031-2. doi: 10.1128/JCM.43.6.3031-3032.2005.
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A modified automated high-throughput mycobacterial interspersed repetitive unit method for genotyping Mycobacterium tuberculosis.一种用于结核分枝杆菌基因分型的改良自动化高通量分枝杆菌散布重复单位方法
Diagn Microbiol Infect Dis. 2005 Dec;53(4):325-7. doi: 10.1016/j.diagmicrobio.2005.05.013. Epub 2005 Nov 9.
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[Application of gene typing approach based on variable-number tandem repeats-mycobacterial interspersed repetitive units in the epidemiological study of Mycobacterium tuberculosis].基于可变数目串联重复序列-分枝杆菌插入重复单元的基因分型方法在结核分枝杆菌流行病学研究中的应用
Zhonghua Jie He He Hu Xi Za Zhi. 2005 May;28(5):342-5.
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Hypervariable loci that enhance the discriminatory ability of newly proposed 15-loci and 24-loci variable-number tandem repeat typing method on Mycobacterium tuberculosis strains predominated by the Beijing family.高变区位点增强了新提出的15位点和24位点可变数目串联重复序列分型方法对以北京家族为主的结核分枝杆菌菌株的鉴别能力。
FEMS Microbiol Lett. 2007 May;270(1):67-74. doi: 10.1111/j.1574-6968.2007.00658.x. Epub 2007 Feb 16.
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[Evaluation on typing of the mycobacterial interspersed repetitive units as a practical approach in gene-typing of the Beijing family].
Zhonghua Liu Xing Bing Xue Za Zhi. 2008 Oct;29(10):1050-1.
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Mycobacterium tuberculosis Beijing genotype and mycobacterial interspersed repetitive unit typing.结核分枝杆菌北京基因型与分枝杆菌插入重复单元分型
J Clin Microbiol. 2006 Apr;44(4):1614; author reply 1614-5. doi: 10.1128/JCM.44.4.1614-1615.2006.
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First molecular epidemiological study of tuberculosis in Benin.贝宁结核病的首次分子流行病学研究。
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引用本文的文献

1
Rapid detection of laboratory cross-contamination with Mycobacterium tuberculosis using multispacer sequence typing.使用多间隔序列分型快速检测结核分枝杆菌实验室交叉污染
BMC Microbiol. 2009 Mar 3;9:47. doi: 10.1186/1471-2180-9-47.
2
Evaluation and strategy for use of MIRU-VNTRplus, a multifunctional database for online analysis of genotyping data and phylogenetic identification of Mycobacterium tuberculosis complex isolates.用于结核分枝杆菌复合群分离株基因分型数据在线分析和系统发育鉴定的多功能数据库MIRU-VNTRplus的评估与应用策略
J Clin Microbiol. 2008 Aug;46(8):2692-9. doi: 10.1128/JCM.00540-08. Epub 2008 Jun 11.