Djelouadji Zoheira, Orehek Jean, Drancourt Michel
Faculté de Médecine, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 6236, IRD 3R198, Université de la Méditerranée, IFR 48, Marseille, France.
BMC Microbiol. 2009 Mar 3;9:47. doi: 10.1186/1471-2180-9-47.
The ability to culture Mycobacterium tuberculosis from clinical specimens serves as the gold standard for the diagnosis of tuberculosis. However, a number of false-positive diagnoses may be due to cross-contamination of such specimens. We herein investigate such episode of cross-contamination by using a technique known as multispacer sequence typing (MST). This technique was applied to six M. tuberculosis isolates prepared within the same laboratory over a two-week period of time.
MST analysis indicated a unique and common sequence profile between a strain isolated from a patient with proven pulmonary tuberculosis and a strain isolated from a patient diagnosed with lung carcinoma. Using this approach, we were able to provide a clear demonstration of laboratory cross-contamination within just four working days. Further epidemiological investigations revealed that the two isolates were processed for culture on the same day.
The application of MST has been demonstrated to serve as a rapid and efficient method to investigate cases of possible cross-contamination with M. tuberculosis.
从临床标本中培养结核分枝杆菌的能力是结核病诊断的金标准。然而，一些假阳性诊断可能是由于此类标本的交叉污染。我们在此使用一种称为多间隔序列分型（MST）的技术来调查此类交叉污染事件。该技术应用于在两周时间内在同一实验室制备的6株结核分枝杆菌分离株。
MST分析表明，从一名确诊为肺结核的患者分离出的菌株与从一名诊断为肺癌的患者分离出的菌株之间存在独特且共同的序列谱。使用这种方法，我们能够在短短四个工作日内清楚地证明实验室交叉污染。进一步的流行病学调查显示，这两株分离株是在同一天进行培养处理的。
已证明应用MST是一种快速有效的方法，可用于调查可能存在的结核分枝杆菌交叉污染病例。
