Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
Plant Physiol. 1992 Oct;100(2):593-6. doi: 10.1104/pp.100.2.593.
After cold acclimation, winter rye (Secale cereale L.) is able to withstand the formation of extracellular ice at freezing temperatures. We now show, for the first time, that cold-acclimated winter rye plants contain endogenously produced antifreeze protein. The protein was extracted from the apoplast of winter rye leaves, where ice forms during freezing. After partial purification, the protein was identified as antifreeze protein because it modified the normal growth pattern of ice crystals and depressed the freezing temperature of water noncolligatively.
经过寒冷驯化后，冬黑麦（Secale cereale L.）能够承受在冰冻温度下形成的细胞外冰。我们现在首次表明，经过寒冷驯化的冬黑麦植物含有内源性产生的抗冻蛋白。该蛋白是从冬黑麦叶片的质外体中提取出来的，在冰冻过程中会在那里形成冰。经过部分纯化后，该蛋白被鉴定为抗冻蛋白，因为它改变了冰晶的正常生长模式并非依数性地降低了水的冰点。
