Institute of Microbiology and Genetics, University of Vienna, Vienna Biocenter, Dr. Bohrgasse 9, 1030 Vienna, Austria.
Plant Physiol. 1992 Oct;100(2):902-7. doi: 10.1104/pp.100.2.902.
The effect of anther-derived substances on pollen function was studied using pollen produced by in vitro culture of immature pollen of tobacco (Nicotiana tabacum L.) and petunia (Petunia hybrida). Addition of conditioned medium consisting of diffusates from in situ matured pollen strongly increased pollen germination frequency and pollen tube growth, as well as seed set after in situ pollination. Thin-layer chromatography and depletion of phenolic substances by Dowex treatment indicated that flavonols are present in the diffusate and may be the active compounds. When added to the germination medium, flavonols (quercetin, kaempferol, myricetin) but not other flavonoids strongly promoted pollen germination frequency and pollen tube growth in vitro. The best results were obtained at very low concentrations of the flavonols (0.15-1.5 mum), indicating a signaling function. The same compounds were also effective when added during pollen development in vitro.
利用烟草（Nicotiana tabacum L.）和矮牵牛（Petunia hybrida）未成熟花粉的离体培养产生的花粉，研究了花粉来源物质对花粉功能的影响。添加由原位成熟花粉的扩散物组成的条件培养基可显著增加原位授粉后花粉的萌发频率和花粉管生长，以及结实率。薄层层析和 Dowex 处理耗尽酚类物质表明，类黄酮存在于扩散物中，可能是活性化合物。当添加到萌发培养基中时，类黄酮（槲皮素、山奈酚、杨梅素）而非其他类黄酮强烈促进花粉体外萌发频率和花粉管生长。在非常低的类黄酮浓度（0.15-1.5 µm）下可获得最佳结果，表明其具有信号功能。当在体外花粉发育过程中添加相同的化合物时，它们也同样有效。
