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A simple and general method for transferring genes into plants.一种将基因转入植物的简单而通用的方法。
Science. 1985 Mar 8;227(4691):1229-31. doi: 10.1126/science.227.4691.1229.
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Genes expressed in the male gametophyte of flowering plants and their isolation.开花植物雄配子体中表达的基因及其分离
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An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin.植物中的一种细胞外基质蛋白：胡萝卜伸展蛋白基因组克隆的特性分析
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Chalcone Synthase Promoters in Petunia Are Active in Pigmented and Unpigmented Cell Types.矮牵牛中的查尔酮合酶启动子在有色素和无色素细胞类型中均有活性。
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A novel purification procedure for chalcone flavanone isomerase from Petunia hybrida and the use of its antibodies to characterize the Po mutation.一种来自矮牵牛的查尔酮黄烷酮异构酶的新型纯化方法及其抗体用于表征Po突变
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矮牵牛中花粉和花药特异性chi启动子：chiA基因的串联启动子调控

Pollen- and anther-specific chi promoters from petunia: tandem promoter regulation of the chiA gene.

作者信息

van Tunen A J, Mur L A, Brouns G S, Rienstra J D, Koes R E, Mol J N

机构信息

Department of Genetics, Vrije Universiteit, Amsterdam, The Netherlands.

出版信息

Plant Cell. 1990 May;2(5):393-401. doi: 10.1105/tpc.2.5.393.

DOI:10.1105/tpc.2.5.393
PMID:2152165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC159896/
Abstract

We have analyzed the spatial and temporal activities of chalcone flavanone isomerase (chi) A and B gene promoters from petunia. To study the tandem promoter regulation of chiA, various chiA promoter fragments were fused with the beta-glucuronidase (GUS) reporter gene. Analysis of transgenic plants containing these chimeric genes provided definitive proof that the chiA coding region is regulated by two distinct promoters (designated PA1 and PA2). We also showed that both promoters can function independently and that the chiA PA1 promoter is expressed in limb (epidermal and parenchyma cells), tube (inner epidermal and parenchyma cells), seed (seed coat, endosperm, and embryo), sepal, leaf, and stem. The use of chiA and chiB promoters in the regulation of anther- and pollen-specific gene expression has been studied. By analyzing transgenic plants containing chimeric genes consisting of chiA and B promoter fragments and the GUS reporter gene, we were able to identify a 0.44-kilobase chiA PA2 promoter fragment that drives pollen-specific gene expression and a 1.75-kilobase chiB PB promoter fragment that confers anther-specific (pollen and tapetum cells) expression to the GUS gene.

摘要

我们分析了矮牵牛查尔酮黄烷酮异构酶（chi）A和B基因启动子的时空活性。为了研究chiA的串联启动子调控，将各种chiA启动子片段与β-葡萄糖醛酸酶（GUS）报告基因融合。对含有这些嵌合基因的转基因植物的分析提供了确凿证据，证明chiA编码区受两个不同的启动子（命名为PA1和PA2）调控。我们还表明，两个启动子都能独立发挥作用，并且chiA PA1启动子在花瓣（表皮和薄壁细胞）、花管（内表皮和薄壁细胞）、种子（种皮、胚乳和胚）、萼片、叶片和茎中表达。我们研究了chiA和chiB启动子在调控花药和花粉特异性基因表达中的应用。通过分析含有由chiA和B启动子片段以及GUS报告基因组成的嵌合基因的转基因植物，我们能够鉴定出一个驱动花粉特异性基因表达的0.44千碱基chiA PA2启动子片段和一个赋予GUS基因花药特异性（花粉和绒毡层细胞）表达的1.75千碱基chiB PB启动子片段。