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免疫细胞化学方法检测烟草叶片中的胞质谷氨酰胺合成酶。

Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods.

机构信息

Centro de Citologia Experimental (Instituto Nacional de Investigação Científica), Universidade do Porto, 4100-Porto, Portugal.

出版信息

Plant Physiol. 1992 Nov;100(3):1591-4. doi: 10.1104/pp.100.3.1591.

DOI:10.1104/pp.100.3.1591
PMID:16653165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1075827/
Abstract

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.

摘要

两个谷氨酰胺合成酶 (GS) 多肽（44 和 39 kD）在来自烟草（Nicotiana tabacum L.）叶片提取物的 Western blot 中被免疫检测到，据报道，这种植物在叶片中仅含有叶绿体 GS。通过免疫细胞化学方法，我们证实了 GS 在叶脉组织细胞的细胞质和叶肉细胞叶绿体中的定位。

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Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods.免疫细胞化学方法检测烟草叶片中的胞质谷氨酰胺合成酶。
Plant Physiol. 1992 Nov;100(3):1591-4. doi: 10.1104/pp.100.3.1591.
2
Immunolocalization of glutamine synthetase in senescing tobacco (Nicotiana tabacum L.) leaves suggests that ammonia assimilation is progressively shifted to the mesophyll cytosol.衰老烟草（烟草）叶片中谷氨酰胺合成酶的免疫定位表明，氨同化作用逐渐转移到叶肉细胞溶质中。
Planta. 2000 Sep;211(4):519-27. doi: 10.1007/s004250000309.
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The chloroplastic glutamine synthetase (GS-2) of tobacco is phosphorylated and associated with 14-3-3 proteins inside the chloroplast.烟草的叶绿体谷氨酰胺合成酶（GS-2）在叶绿体内被磷酸化并与14-3-3蛋白相关联。
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Chloroplast and cytosolic glutamine synthetase are encoded by homologous nuclear genes which are differentially expressed in vivo.叶绿体和胞质谷氨酰胺合成酶由同源核基因编码，这些基因在体内差异表达。
J Biol Chem. 1988 Jul 15;263(20):9651-7.
5
Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.体外培养的烟草（Nicotiana tabacum）植株中自然叶片衰老的特征分析。
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Expression of a ferredoxin-dependent glutamate synthase gene in mesophyll and vascular cells and functions of the enzyme in ammonium assimilation in Nicotiana tabacum (L.).铁氧还蛋白依赖性谷氨酸合酶基因在烟草叶肉细胞和维管束细胞中的表达及其在铵同化中的功能
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J Exp Bot. 2001 May;52(358):1071-81. doi: 10.1093/jexbot/52.358.1071.
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Glutamine Synthetase of Nicotiana plumbaginifolia: Cloning and in Vivo Expression.菸草磷酸吡哆醛谷氨酸合成酶的克隆与体内表达。
Plant Physiol. 1987 Jun;84(2):366-73. doi: 10.1104/pp.84.2.366.
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Localization of tobacco cytosolic glutamine synthetase enzymes and the corresponding transcripts shows organ- and cell-specific patterns of protein synthesis and gene expression.烟草胞质谷氨酰胺合成酶的定位以及相应的转录本显示出蛋白质合成和基因表达的器官特异性和细胞特异性模式。
Plant Mol Biol. 1996 Jul;31(4):803-17. doi: 10.1007/BF00019468.
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本文引用的文献

1
Glutamine Synthetase of Nicotiana plumbaginifolia: Cloning and in Vivo Expression.菸草磷酸吡哆醛谷氨酸合成酶的克隆与体内表达。
Plant Physiol. 1987 Jun;84(2):366-73. doi: 10.1104/pp.84.2.366.
2
Multiple Subunit Composition of Chloroplastic Glutamine Synthetase of Nicotiana tabacum L.叶绿体谷氨酰胺合成酶的多亚基组成烟草Nicotiana tabacum L.
Plant Physiol. 1984 Feb;74(2):448-50. doi: 10.1104/pp.74.2.448.
3
Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf.
乙醇脱水及其对 Chlorogloeopsis fritschii 和 Chlorella pyrenoidosa 膜结合酶的影响。
World J Microbiol Biotechnol. 1994 Mar;10(2):187-90. doi: 10.1007/BF00360884.
4
Novel aspects of glutamine synthetase (GS) regulation revealed by a detailed expression analysis of the entire GS gene family of Medicago truncatula under different physiological conditions.通过对不同生理条件下蒺藜苜蓿（Medicago truncatula）整个谷氨酰胺合成酶（GS）基因家族的详细表达分析，揭示了 GS 调节的新方面。
BMC Plant Biol. 2013 Sep 21;13:137. doi: 10.1186/1471-2229-13-137.
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Primary N-assimilation into Amino Acids in Arabidopsis.拟南芥中氮素向氨基酸的初级同化
Arabidopsis Book. 2003;2:e0010. doi: 10.1199/tab.0010. Epub 2003 Sep 30.
6
Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco.谷氨酰胺合成酶-谷氨酸合酶途径和谷氨酸脱氢酶在烟草库-源氮循环中发挥着不同作用。
Plant Physiol. 2006 Feb;140(2):444-56. doi: 10.1104/pp.105.071910. Epub 2006 Jan 11.
7
Biochemical and molecular characterization of transgenic Lotus japonicus plants constitutively over-expressing a cytosolic glutamine synthetase gene.组成型过表达胞质谷氨酰胺合成酶基因的转基因日本百脉根植株的生化及分子特性分析
Planta. 2004 Sep;219(5):807-18. doi: 10.1007/s00425-004-1292-8. Epub 2004 Jun 10.
8
Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids.蒺藜苜蓿质体定位谷氨酰胺合成酶的表达。根瘤中前体的积累揭示了蛋白质导入质体水平上的体内调控。
Plant Physiol. 2003 May;132(1):390-9. doi: 10.1104/pp.102.016675.
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Plant Physiol. 1994 Feb;104(2):425-430. doi: 10.1104/pp.104.2.425.
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Plant Physiol. 1995 Mar;107(3):757-763. doi: 10.1104/pp.107.3.757.
高等植物的谷氨酰胺合成酶：与它们可能的生理作用及其在叶片中的区室化相关的特定同工型含量的证据。
Plant Physiol. 1983 May;72(1):22-5. doi: 10.1104/pp.72.1.22.
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Glutamine synthetase of Phaseolus vulgaris L.: organ-specific expression of a multigene family.菜豆谷氨酰胺合成酶：一个多基因家族的器官特异性表达
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase.转基因植物中的细胞特异性表达揭示了叶绿体和胞质谷氨酰胺合成酶的非重叠作用。
Proc Natl Acad Sci U S A. 1990 May;87(9):3459-63. doi: 10.1073/pnas.87.9.3459.