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叶绿体谷氨酰胺合成酶的多亚基组成烟草Nicotiana tabacum L.

Multiple Subunit Composition of Chloroplastic Glutamine Synthetase of Nicotiana tabacum L.

机构信息

Physiologie Végétale Métabolique, ERA au CNRS 799, Université de Paris-Sud, Bât. 430, 91405 Orsay Cedex, France.

出版信息

Plant Physiol. 1984 Feb;74(2):448-50. doi: 10.1104/pp.74.2.448.

DOI:10.1104/pp.74.2.448
PMID:16663441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1066701/
Abstract

Chloroplastic glutamine synthetase from tobacco leaves (Nicotiana tabacum L. var Xanthi) was purified to homogeneity. By using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high performance liquid chromatography, a single subunit was identified with a molecular weight of 45,000 daltons. However the native protein seems to be composed of four different subunits which can be separated by isoelectrofocusing. It is suggested that different genes with eventual posttranslational and/or posttranscriptional modifications may control the synthesis of the chloroplastic glutamine synthetase.

摘要

叶绿体谷氨酰胺合成酶从烟草叶（烟草。变种黄）被纯化到均质。通过使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和高效液相色谱，一个单一的亚基被鉴定具有分子量为 45000 道尔顿。然而，天然蛋白质似乎由四个不同的亚基组成，这些亚基可以通过等电聚焦分离。这表明不同的基因可能通过翻译后和/或转录后修饰来控制叶绿体谷氨酰胺合成酶的合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0052/1066701/64cd93393663/plntphys00571-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0052/1066701/64cd93393663/plntphys00571-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0052/1066701/64cd93393663/plntphys00571-0265-a.jpg

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本文引用的文献

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Plant Physiol. 1983 May;72(1):22-5. doi: 10.1104/pp.72.1.22.
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Isolation and Immunochemical Characterization of Plant Glutamine Synthetase in Alfalfa (Medicago sativa L.) Nodules.苜蓿（Medicago sativa L.）根瘤中植物谷氨酰胺合成酶的分离与免疫化学特性。
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Glutamine Synthetase in Spinach Leaves : IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION.
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