Department of Botany, University of Florida, Gainesville, Florida.
Plant Physiol. 1966 Feb;41(2):197-202. doi: 10.1104/pp.41.2.197.
Etiolated maize and soybean seedlings were grown for several days in atmospheres enriched with O(18). Hydroxyproline subsequently isolated from the seedlings by column and thin-layer chromatography was labeled with excess O(18), but proline was not. Control experiments in which seedlings were grown in H(2)O(18) and unlabeled atmospheres demonstrated that neither proline nor hydroxyproline was labeled with excess O(18). It was concluded that oxygen fixation is an essential feature of hydroxyproline biosynthesis in these seedlings, and that the hydroxyl oxygen atom in hydroxyproline is derived from molecular oxygen and not from water; similar results have been reported previously for sycamore cell suspensions.
将玉米和大豆的黄化幼苗在富含 O(18)的大气中生长数天。随后通过柱层析和薄层层析从幼苗中分离出羟脯氨酸，并对其进行过量 O(18)标记，但脯氨酸没有被标记。在以 H(2)O(18)和未标记大气中生长的幼苗的对照实验中表明，脯氨酸和羟脯氨酸均未被过量 O(18)标记。因此得出结论，氧固定是这些幼苗中羟脯氨酸生物合成的一个基本特征，并且羟脯氨酸中的羟基氧原子来自于分子氧而不是水；先前已报道过类似的结果，适用于悬铃木细胞悬浮液。
