一种用于测量放射性离子穿透完整植物根系的系统。
A system for measuring penetration of radioactive ions across roots of intact plants.
机构信息
Plant Science Department, University of Connecticut, Storrs.
出版信息
Plant Physiol. 1966 Feb;41(2):244-7. doi: 10.1104/pp.41.2.244.
A technique was developed for estimating penetration of P(32) across roots of intact plants (Phaseolus vulgaris) by measuring the level of isotope in the xylem stream. Penetration was defined as movement from the root surface to the xylem sap. The xylem sap measurement for P(32) was made in the stem as the material ascended the plant in the transpiration stream. Stems were held near 0 degrees to arrest metabolic concentration of isotope adjacent to the xylem column. A 3 layer environment control system was constructed to allow stem chilling in a manner that would not interfere with the environments of the roots or foliage. Despite these precautions, some extra-xylary build-up of (32)P occurred in the chilled stem. The mathematical function of the extra-xylary fraction was derived, and the difference between this value and total P(32) in the stem represented xylem sap isotope.
开发了一种技术，通过测量木质部流中同位素的水平来估计完整植物（菜豆）根系中 P(32) 的穿透情况。穿透被定义为从根表面到木质部汁液的移动。木质部汁液中 P(32)的测量是在茎中进行的，因为材料在蒸腾流中上升到植物中。茎被保持在接近 0 度的位置，以阻止同位素在木质部柱附近的代谢浓缩。构建了一个 3 层环境控制系统，以允许茎在不干扰根部或叶片环境的情况下冷却。尽管采取了这些预防措施，但在冷却的茎中仍发生了一些木质部以外的（32）P 积累。导出了额外木质部部分的数学函数，该值与茎中总 P(32)之间的差异代表木质部汁液同位素。
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