College of Horticulture, China Agricultural University, Beijing, China.
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, National Engineering Research Center for Vegetables, Beijing, China.
Sci Rep. 2017 Mar 29;7(1):503. doi: 10.1038/s41598-017-00566-1.
Seed germination is a critical and complex process in the plant life cycle. Although previous studies have found that melatonin can promote seed germination under salt stress, the involvement of melatonin in the regulation of proteomic changes remains poorly understood. In this study, a total of 157 proteins were significantly influenced (ratio ≥ 2 or ≤ -2) by melatonin during seed germination under salt stress using a label-free quantitative technique. Our GO analysis revealed that several pathways were obviously regulated by melatonin, including ribosome biosynthesis, lipid metabolism, carbohydrate metabolism, and storage protein degradation. Not only stress-tolerant proteins but also proteins that produce ATP as part of glycolysis, the citric acid cycle, and the glyoxylate cycle were upregulated by melatonin. Overall, this study provides new evidence that melatonin alleviates the inhibitory effects of NaCl stress on seed germination by promoting energy production. This study is the first to provide insights at the proteomic level into the molecular mechanism of melatonin in response to salt stress in cucumber seeds. This may be helpful to further understand the role of melatonin in cucumber seed germination under stress conditions.
种子萌发是植物生命周期中的一个关键而复杂的过程。尽管先前的研究发现褪黑素可以在盐胁迫下促进种子萌发，但褪黑素在调节蛋白质组变化方面的作用仍知之甚少。在这项研究中，使用无标记定量技术，我们发现褪黑素在盐胁迫下的种子萌发过程中，总共影响了 157 种显著变化的蛋白质（比值≥2 或≤-2）。我们的 GO 分析表明，褪黑素明显调节了几个途径，包括核糖体生物合成、脂质代谢、碳水化合物代谢和储存蛋白降解。褪黑素不仅上调了应激耐受蛋白，还上调了产生 ATP 的蛋白，这些蛋白参与糖酵解、柠檬酸循环和乙醛酸循环。总的来说，这项研究提供了新的证据，表明褪黑素通过促进能量产生来缓解 NaCl 胁迫对种子萌发的抑制作用。这项研究首次在蛋白质组水平上揭示了褪黑素在黄瓜种子响应盐胁迫中的分子机制。这可能有助于进一步了解褪黑素在胁迫条件下对黄瓜种子萌发的作用。
