仅在蜡样芽孢杆菌T型孢子生长期间限制³H-胸腺嘧啶核苷的放射性掺入脱氧核糖核酸的方法
Method for restricting incorporation of radioactivity from 3 H-thymidine into deoxyribonucleic acid only during outgrowth of spores of Bacillus cereus T.
作者信息
Rana R S, Halvorson H O
出版信息
J Bacteriol. 1972 Feb;109(2):599-605. doi: 10.1128/jb.109.2.599-605.1972.
Abstract
When heat-activated spores of Bacillus cereus T (thy(-)) were germinated and grown in medium containing (3)H-thymidine, a significant amount of radioactivity was incorporated into ribonucleic acid and deoxyribonucleic acid (DNA). A method was developed to restrict the incorporation of radioactivity from (3)H-thymidine into DNA only. This was accomplished by labeling the cells with (3)H-thymidine in the presence of 2 mg of 2-deoxyadenosine per ml, 250 mug each of uracil, cytosine, and guanosine per ml, and 500 mug of adenosine per ml. Under these conditions, 97% of the radioactivity incorporated into cold trichloroacetic acid-insoluble material was associated with DNA only. In the absence of these compounds, DNA contained only 72% of the total radioactivity incorporated into cold acid-insoluble material.
摘要
当蜡样芽孢杆菌T（thy(-)）的热激活孢子在含有³H-胸腺嘧啶核苷的培养基中萌发并生长时，大量放射性被掺入核糖核酸和脱氧核糖核酸（DNA）中。开发了一种方法来仅限制³H-胸腺嘧啶核苷的放射性掺入DNA中。这是通过在每毫升含有2毫克2-脱氧腺苷、每毫升250微克尿嘧啶、胞嘧啶和鸟苷以及每毫升500微克腺苷的存在下用³H-胸腺嘧啶核苷标记细胞来实现的。在这些条件下，掺入冷三氯乙酸不溶性物质中的放射性的97%仅与DNA相关。在没有这些化合物的情况下，DNA仅含有掺入冷酸不溶性物质中的总放射性的72%。
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