Stafford H A
Biology Department, Reed College, Portland, Oregon 97202.
Plant Physiol. 1968 Mar;43(3):318-26. doi: 10.1104/pp.43.3.318.
The initiation and subsequent growth of adventitious roots in excised first internodes of Sorghum vulgare var. Wheatland milo were studied to determine the effect of these processes on anthocyanin biosyntheses. Segmentation of the internodes inhibited both adventitious root growth and accumulation of cyanidin equally in all segments; these results can be interpreted as a common requirement for bidirectional longitudinal transport. The presence of the coleoptile, especially in the absence of the base of the internode, inhibited the growth of the roots, but increased the number of root initials. High intensities of white and blue light which induced cyanidin synthesis slightly decreased adventitious root growth. Anaerobic conditions produced by solution infiltration strongly inhibited the growth of adventitious roots and greatly increased the accumulation of apigeninidin and luteolinidin. Addition of indoleacetic acid, kinetin and cofactors such as pyridoxine produced effects on the initiation and subsequent growth of these roots similar to those effects reported in the literature. But unlike root formation in hypocotyls, the initiation of adventitious roots in Sorghum internodes was not always directly correlated with the accumulation of anthocyanins, and the subsequent growth of these roots was frequently inversely correlated with some of the anthocyanin biosyntheses. The possible nature of these correlations is discussed. Comparisons are made with related Sorghum lines and mutants.
研究了高粱品种惠特兰 Milo 离体第一节间不定根的起始及随后的生长情况，以确定这些过程对花青素生物合成的影响。节间分割在所有节段中均同等程度地抑制了不定根生长和花青素的积累；这些结果可解释为双向纵向运输的共同需求。胚芽鞘的存在，尤其是在节间基部缺失的情况下，抑制了根的生长，但增加了根原基的数量。诱导花青素合成的高强度白光和蓝光略微降低了不定根的生长。溶液浸润产生的厌氧条件强烈抑制了不定根的生长，并大大增加了芹菜素idin 和木犀草素idin 的积累。添加吲哚乙酸、激动素和诸如吡哆醇等辅因子对这些根的起始及随后的生长产生的影响与文献报道的影响相似。但与下胚轴中根的形成不同，高粱节间不定根的起始并不总是与花青素的积累直接相关，并且这些根的随后生长经常与某些花青素生物合成呈负相关。讨论了这些相关性的可能性质。与相关高粱品系和突变体进行了比较。
