南瓜中易位水苏糖的标记模式。

Department of Botany, The Ohio State University, Columbus, Ohio 43210.

Plant Physiol. 1968 Oct;43(10):1631-6. doi: 10.1104/pp.43.10.1631.

One mature blade of each squash plant was continuously labeled with (14)CO(2) for 15, 30, or 70 minutes in light. The ethanol soluble materials from serial sections of petioles were extracted and separated by paper chromatography. The ratios of label in the various components of this fraction were determined. Stachyose, which contained the major portion of the label of this fraction, was hydrolyzed and the resultant hydrolysate was separated by paper chromatography. Specific activities of the hexoses derived from stachyose were determined. It was found that the glucose and fructose moieties of stachyose became labeled at the same rates; however, the galactose moiety became labeled more rapidly.

在光照条件下，对每株南瓜植株的一片成熟叶片连续用¹⁴CO₂标记15、30或70分钟。从叶柄的连续切片中提取乙醇可溶物质，并通过纸色谱法进行分离。测定该部分各组分中的标记比率。水苏糖含有该部分标记的主要部分，将其水解，所得水解产物通过纸色谱法分离。测定水苏糖衍生的己糖的比活性。发现水苏糖的葡萄糖和果糖部分以相同的速率被标记；然而，半乳糖部分被标记的速度更快。