Department of Biochemistry, University of Arizona, Tucson, Arizona 85721.
Plant Physiol. 1992 Feb;98(2):753-6. doi: 10.1104/pp.98.2.753.
Phloem sap from cotton (Gossypium hirsutum L.) was collected from young and mature leaves by the aphid-stylet technique. Exudate was analyzed for carbohydrates by HPLC using sensitive pulsed amperometric detection. The predominant carbohydrate present (>90%) was identified as sucrose. A second, unidentified compound that was not one of the more commonly translocated sugars was detected in mature leaves. Carbohydrates in honeydew produced by the sweet-potato whitefly (Bemisia tabaci [Genn.]) feeding on cotton were sucrose, glucose, fructose, trehalulose, and a series of oligosaccharides.
采用蚜虫刺吸技术从棉花(Gossypium hirsutum L.)的幼叶和成熟叶中收集韧皮部汁液。采用高效液相色谱法(HPLC)和灵敏的脉冲安培检测法分析渗出物中的碳水化合物。主要存在的碳水化合物(>90%)被鉴定为蔗糖。在成熟叶片中还检测到另一种未鉴定的化合物,它不是更常见的可转移糖之一。甘薯粉虱(Bemisia tabaci [Genn.])在棉花上取食产生的蜜露中的碳水化合物为蔗糖、葡萄糖、果糖、海藻糖和一系列低聚糖。