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高效液相色谱法分析棉粉虱取食棉花后泌蜜及棉株韧皮部中碳水化合物

High Performance Liquid Chromatography Analysis of Carbohydrates of Cotton-Phloem Sap and of Honeydew Produced by Bemisia tabaci Feeding on Cotton.

机构信息

Department of Biochemistry, University of Arizona, Tucson, Arizona 85721.

出版信息

Plant Physiol. 1992 Feb;98(2):753-6. doi: 10.1104/pp.98.2.753.

Abstract

Phloem sap from cotton (Gossypium hirsutum L.) was collected from young and mature leaves by the aphid-stylet technique. Exudate was analyzed for carbohydrates by HPLC using sensitive pulsed amperometric detection. The predominant carbohydrate present (>90%) was identified as sucrose. A second, unidentified compound that was not one of the more commonly translocated sugars was detected in mature leaves. Carbohydrates in honeydew produced by the sweet-potato whitefly (Bemisia tabaci [Genn.]) feeding on cotton were sucrose, glucose, fructose, trehalulose, and a series of oligosaccharides.

摘要

采用蚜虫刺吸技术从棉花(Gossypium hirsutum L.)的幼叶和成熟叶中收集韧皮部汁液。采用高效液相色谱法(HPLC)和灵敏的脉冲安培检测法分析渗出物中的碳水化合物。主要存在的碳水化合物(>90%)被鉴定为蔗糖。在成熟叶片中还检测到另一种未鉴定的化合物,它不是更常见的可转移糖之一。甘薯粉虱(Bemisia tabaci [Genn.])在棉花上取食产生的蜜露中的碳水化合物为蔗糖、葡萄糖、果糖、海藻糖和一系列低聚糖。

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