Samuel A R, Knowles N J, Samuel G D, Crowther J R
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
Biologicals. 1991 Oct;19(4):299-310. doi: 10.1016/s1045-1056(05)80019-3.
A trapping enzyme-linked immunosorbent assay (ELISA) has been evaluated for the differentiation of foot-and-mouth disease virus (FMDV) strains using a panel of seven anti-serotype O monoclonal antibodies (MAbs). The variation of results within and between tests performed on the same day and on different days was examined using three strains of FMDV. Criteria for establishing antigenic differences between the strains as defined by the individual MAbs are proposed based on the variability measured, which can be used as standards by workers performing this test with other MAbs and FMDV strains.
已使用一组七种抗O型口蹄疫病毒单克隆抗体（MAb），对一种捕获酶联免疫吸附测定（ELISA）进行评估，以区分口蹄疫病毒（FMDV）毒株。使用三株FMDV，检测了同一天以及不同日期进行的测试内部和之间结果的差异。根据所测得的变异性，提出了由各个MAb定义的毒株之间抗原差异的判定标准，可供使用其他MAb和FMDV毒株进行该测试的人员用作标准。
