Department of Botany, University of Maryland, College Park, Maryland 20740.
Plant Physiol. 1969 Oct;44(10):1413-6. doi: 10.1104/pp.44.10.1413.
Qualitative and quantitative analyses were made of the major fatty acids of Chlorella fusca, Shihira and Krauss, in both autotrophic and heterotrophic culture. Cells grown heterotrophically were subjected to CO(2) concentrations as great as 40%-in-air.The major fatty acids of autotrophic cells grown under a 1% environmental concentration of CO(2), in order of concentration were 16:0, 18:3, 16:4, 18:2, 18:1, and 16:3. The analysis of heterotrophic cells at 1% CO(2), by comparison, indicated a complete absence of 16:4, reduced amounts of 18:3, and increased amounts of the other major acids.An increase in the environmental concentration of CO(2) (from 1%-30%) over the heterotrophic cultures produced a 40% increase in total lipids and nearly a 50% increase in fatty acids. Palmitic acid (16:0) and 18:1 largely account for the fatty acid change by increasing from 12 mg to 33 mg/g dry wt and 8 mg to 17 mg/g dry wt respectively. Possible explanations for this enhanced synthesis of fatty acids are suggested.
对小球藻、石莼和克劳斯绿藻在自养和异养培养中的主要脂肪酸进行了定性和定量分析。在异养培养中，细胞接受高达 40%的大气 CO2浓度。在环境 CO2浓度为 1%的条件下生长的自养细胞中，按浓度顺序排列的主要脂肪酸为 16:0、18:3、16:4、18:2、18:1 和 16:3。相比之下，对 1%CO2 下的异养细胞进行分析表明，16:4 完全缺失，18:3 含量减少，其他主要酸含量增加。在异养培养中，环境 CO2 浓度（从 1%增加到 30%）增加了 40%的总脂质和近 50%的脂肪酸。棕榈酸（16:0）和 18:1 通过从 12mg/g 干重增加到 33mg/g 干重和从 8mg/g 干重增加到 17mg/g 干重，分别对脂肪酸的变化有较大影响。对这种脂肪酸合成增强的可能解释进行了探讨。
