School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China.
Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487, USA.
Bioresour Technol. 2015 Sep;191:281-90. doi: 10.1016/j.biortech.2015.04.116. Epub 2015 May 9.
The fermentation process for high yields of fatty acid and neutral lipid production from cassava bagasse hydrolysate (CBH) was developed by heterotrophic Chlorella protothecoides. An efficient single-step enzymatic hydrolysis of cassava bagasse (CB) by cellulase was firstly developed to produce >30 g/L of reducing sugars. The concentrated CBH was subsequently applied in a batch culture, producing 7.9 g/L of dry biomass with yield of 0.44 g/g reducing sugar and 34.3 wt% of fatty acids and 48.6 wt% of neutral lipids. Furthermore, fed-batch fermentation using CBH achieved higher yields of fatty acids (41.0 wt% and a titer of 5.83 g/L) and neutral lipids (58.4 wt% and yield of 0.22 g/g reducing sugar). Additionally, the fatty acid profile analysis showed that the intercellular lipid was suitable to prepare high-quality biodiesel. This study demonstrated the feasibility of using CBH as low-cost feedstock to produce crude algal oil for sustainable biodiesel production.
木薯渣水解液（CBH）高产脂肪酸和中性脂质生产的发酵工艺是通过异养小球藻属开发的。首先通过纤维素酶对木薯渣（CB）进行高效的一步酶解，以产生>30 g/L 的还原糖。随后将浓缩的 CBH 应用于分批培养，生产出 7.9 g/L 的干生物质，还原糖得率为 0.44 g/g，脂肪酸含量为 34.3 wt%，中性脂质含量为 48.6 wt%。此外，使用 CBH 的补料分批发酵实现了更高的脂肪酸产量（41.0 wt%和 5.83 g/L 的浓度）和中性脂质产量（58.4 wt%和 0.22 g/g 还原糖的得率）。此外，脂肪酸谱分析表明，细胞间脂质适合制备高质量的生物柴油。本研究表明，使用 CBH 作为低成本原料生产粗藻类油用于可持续生物柴油生产是可行的。
