不同培养条件下三角褐指藻的生长及ω-3脂肪酸产量
Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions.
作者信息
Yongmanitchai W, Ward O P
机构信息
Department of Biology, University of Waterloo, Ontario, Canada.
出版信息
Appl Environ Microbiol. 1991 Feb;57(2):419-25. doi: 10.1128/aem.57.2.419-425.1991.
Abstract
Detailed studies were carried out on the effects of nitrogen source, phosphate, sodium chloride, growth factors, precursors, CO2, temperature, initial pH, and inoculum size on biomass and eicosapentaenoic acid (EPA) production by Phaeodactylum tricornutum. The EPA content of total fatty acids increased with increasing concentrations of nitrate and urea. Sodium chloride was not required for growth or EPA production. While vitamins B1 and B12 did not affect growth significantly, EPA yield was increased by 65% by B12 supplementation. Maximum EPA production occurred when the air gassing supply was supplemented with 1% CO2. Optimum culture temperature and initial pH for EPA production were 21.5 to 23 degrees C and 7.6, respectively. EPA yields of up to 133 mg/liter of culture were observed. EPA constituted up to 30 to 40% of total fatty acids.
摘要
对氮源、磷酸盐、氯化钠、生长因子、前体物质、二氧化碳、温度、初始pH值和接种量对三角褐指藻生物量和二十碳五烯酸（EPA）产量的影响进行了详细研究。总脂肪酸中的EPA含量随着硝酸盐和尿素浓度的增加而增加。生长或EPA生产不需要氯化钠。虽然维生素B1和B12对生长没有显著影响，但补充B12可使EPA产量提高65%。当空气通气供应补充1%二氧化碳时，EPA产量最高。EPA生产的最佳培养温度和初始pH值分别为21.5至23摄氏度和7.6。观察到EPA产量高达133毫克/升培养物。EPA占总脂肪酸的比例高达30%至40%。
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