Department of Food Science and Technology, University of California, Davis, California 95616.
Plant Physiol. 1969 Nov;44(11):1560-5. doi: 10.1104/pp.44.11.1560.
While the total amount of proteolytic enzyme activity in the latex from Ficus carica varieties Kadota and Black Mission showed seasonal variation and was different between the 2 crops of figs in the latex collected at the same time and from the same tree, there is no difference in the number of multiple molecular forms of ficin present as demonstrated by chromatography on carboxymethyl-cellulose. The number of multiple forms of ficin is also the same, with 1 exception, among samples of latex which have been collected with no protection against proteolysis, samples which have been collected directly into sodium p-chloromercuribenzoate to inhibit all proteolysis and samples which have been held at an elevated temperature so as to encourage proteolysis. Evidence is presented to support the proposal that component C of Ficus carica variety Kadota is not present in the original latex but that it is produced from component D during the purification procedure. The data support the hypothesis that, with the possible exception of 1 component, the multiple molecular forms of ficin are not the result of artifacts produced by autolysis during collection, storage and purification of the sample.
虽然卡多塔和黑使命两种番荔枝乳胶中的蛋白水解酶总活性表现出季节性变化，并且在同一时间和同一树上采集的两种番荔枝乳胶之间存在差异，但在羧甲基纤维素上的色谱分析表明，ficin 的多种分子形式的数量没有差异。在没有防止蛋白水解的情况下采集的乳胶样品、直接收集到对所有蛋白水解有抑制作用的对氯汞苯甲酸的钠盐中的乳胶样品以及置于升高温度下以促进蛋白水解的乳胶样品中，ficin 的多种形式的数量也是相同的，只有 1 个例外。有证据表明，卡多塔番荔枝品种的 C 组分不存在于原始乳胶中，而是在纯化过程中由 D 组分产生的。这些数据支持了这样一种假设，即除了 1 种组分之外，ficin 的多种分子形式不是在收集、储存和纯化样品过程中自溶产生的假象的结果。
