来自埃及乳胶的纤溶丝氨酸蛋白酶对促凝血剂无花果蛋白酶的矛盾作用。
A contradictory action of procoagulant ficin by a fibrinolytic serine protease from Egyptian latex.
作者信息
Hamed Mohamed B, El-Badry Mohamed O, Kandil Eman I, Borai Ibrahim H, Fahmy Afaf S
机构信息
Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokii, Cairo, Egypt.
Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
出版信息
Biotechnol Rep (Amst). 2020 Jun 20;27:e00492. doi: 10.1016/j.btre.2020.e00492. eCollection 2020 Sep.
is one of the most popular and edible plants. Its trees emanate latex of high medical importance. The well-studied procoagulant effect of ficin is a hallmark of this latex which protrudes an interesting question of how can the plant control this effect? In the present work, we purified and characterized a serine protease (FPIII) with fibrinolytic activity from latex and study the anticoagulant character of the latex. FPIII was inhibited by PMSF and its molecular weight was 48 kDa. The optimum pH and temperature of FPIII were detected at 8.5 and 60 °C, respectively. The activation energy of FPIII was 7 kcal/mol and was thermal stable up to 60 °C. FPIII tended to hydrolyze different protein substrates and showed a good catalytic efficiency (K/K). The anticoagulant effects and fibrinogenolytic activities of latex crude extract and FPIII were detected, which controls the procoagulant effect of ficin.
是最受欢迎的可食用植物之一。它的树分泌出具有高度医学重要性的乳胶。对无花果蛋白酶促凝血作用的深入研究是这种乳胶的一个标志，这引出了一个有趣的问题：植物如何控制这种作用？在本研究中，我们从乳胶中纯化并鉴定了一种具有纤溶活性的丝氨酸蛋白酶（FPIII），并研究了乳胶的抗凝特性。FPIII被苯甲基磺酰氟（PMSF）抑制，其分子量为48 kDa。FPIII的最适pH值和温度分别为8.5和60°C。FPIII的活化能为7千卡/摩尔，在60°C以下热稳定。FPIII倾向于水解不同的蛋白质底物，并表现出良好的催化效率（K/K）。检测了乳胶粗提物和FPIII的抗凝作用和纤维蛋白原溶解活性，它们控制了无花果蛋白酶的促凝血作用。
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