Kleha J F, Devesly P, Johns A
Department of Pharmacology, Berlex Laboratories, Cedar Knolls, New Jersey 07927.
Br J Pharmacol. 1991 Oct;104(2):287-8. doi: 10.1111/j.1476-5381.1991.tb12421.x.
The effects of the lectins, wheat germ agglutinin (WGA) and concanavalin A (Con A) on endothelium intact pre-contracted rabbit aorta were investigated. WGA (8.3 microM) produced endothelium-dependent relaxations, while Con A (4.3 microM) produced a partially endothelium-dependent relaxation. The endothelium-dependent relaxations to WGA were completely reversed by N-acetylglucosamine, haemoglobin and methylene blue and were partially reversed by NG-monomethyl-L-arginine (L-NMMA). It is suggested that WGA works as an agonist in releasing endothelium-derived relaxing factor (EDRF) from the endothelium of rabbit aorta by interacting with a glycosylated receptor on endothelial cells.
研究了凝集素、麦胚凝集素（WGA）和伴刀豆球蛋白A（Con A）对内皮完整的预收缩兔主动脉的影响。WGA（8.3微摩尔）产生内皮依赖性舒张，而Con A（4.3微摩尔）产生部分内皮依赖性舒张。WGA引起的内皮依赖性舒张可被N-乙酰葡糖胺、血红蛋白和亚甲蓝完全逆转，并被NG-单甲基-L-精氨酸（L-NMMA）部分逆转。提示WGA通过与内皮细胞上的糖基化受体相互作用，作为一种激动剂从兔主动脉内皮释放内皮源性舒张因子（EDRF）。
