受体介导的牛主动脉内皮细胞释放内皮衍生舒张因子和前列环素是偶联的。
Receptor-mediated release of endothelium-derived relaxing factor and prostacyclin from bovine aortic endothelial cells is coupled.
作者信息
de Nucci G, Gryglewski R J, Warner T D, Vane J R
机构信息
William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 1988 Apr;85(7):2334-8. doi: 10.1073/pnas.85.7.2334.
Abstract
Bovine aortic endothelial cells were grown on microcarrier beads and were perfused with Krebs-Ringer solution. Endothelium-derived relaxing factor (EDRF) was bioassayed on a cascade of four strips of rabbit aorta, and prostacyclin was analyzed by RIA of 6-oxo-prostaglandin F1 alpha. The endothelial cells released EDRF and prostacyclin when stimulated with bradykinin and its analogues, or with ADP, ATP, arachidonic acid, and phospholipase C (phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3). The detection of EDRF was potentiated by superoxide dismutase, and the relaxation of rabbit aortic strips induced by EDRF was antagonized by methylene blue. The release of EDRF and prostacyclin was inhibited by phorbol myristate acetate, R59022 (a diacylglycerol kinase inhibitor), and gentamycin. We suggest that the release of EDRF and prostacyclin is coupled and the initial common step is activation of a phospholipase C.
摘要
牛主动脉内皮细胞生长在微载体珠上，并用 Krebs-Ringer 溶液灌注。内皮衍生舒张因子（EDRF）在由四条兔主动脉条组成的级联装置上进行生物测定，前列环素通过 6-氧代前列腺素 F1α 的放射免疫分析进行检测。当用缓激肽及其类似物、或用 ADP、ATP、花生四烯酸和磷脂酶 C（磷脂酰胆碱胆碱磷酸水解酶，EC 3.1.4.3）刺激时，内皮细胞释放 EDRF 和前列环素。超氧化物歧化酶增强了 EDRF 的检测，亚甲蓝拮抗了 EDRF 诱导的兔主动脉条舒张。佛波醇肉豆蔻酸酯、R59022（一种二酰基甘油激酶抑制剂）和庆大霉素抑制了 EDRF 和前列环素的释放。我们认为 EDRF 和前列环素的释放是偶联的，最初的共同步骤是磷脂酶 C 的激活。
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