Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, Australia.
Plant Physiol. 1970 May;45(5):608-11. doi: 10.1104/pp.45.5.608.
DNA-dependent RNA polymerase activity has been found in both the nuclei and etioplasts of dark-grown pea seedlings (Pisum sativum). Although these enzymes had similar over-all characteristics with respect to substrate, pH, and inhibitor responses, they could be distinguished by their different sensitivities to sonication.Brief irradiation of the seedlings with red light resulted in an increase in the activity of both the nuclear and plastid enzymes, but the time course of this response showed it to be too slow to be considered an early result of phytochrome control of metabolism. Far red light following red treatment largely prevented the increase in the activity of the nuclear enzyme. The plastid enzyme responded to far red light in a manner similar to its response to red light so that no reversal was observed when far red treatment followed red.
在黑暗中生长的豌豆幼苗（Pisum sativum）的细胞核和前质体中均发现了依赖 DNA 的 RNA 聚合酶活性。尽管这些酶在底物、pH 和抑制剂反应方面具有相似的整体特征，但它们可以通过对超声的不同敏感性来区分。幼苗用红光短暂照射会导致核酶和质体酶的活性增加，但这种反应的时间进程表明它太慢，不能被认为是光质体对代谢控制的早期结果。红光处理后的远红光很大程度上阻止了核酶活性的增加。质体酶对远红光的反应与对红光的反应相似，因此当远红光处理紧随红光处理时，没有观察到逆转。
