Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, P.O. Box 109, Canberra City, 2601, Australia.
Plant Physiol. 1970 Sep;46(3):437-41. doi: 10.1104/pp.46.3.437.
When pea etioplast preparations were treated with Triton X-100, the membranes disappeared, the pigments were solubilized, and the organelles appeared to disintegrate. Low speed centrifugation (2000g) of the preparations following treatment with Triton X-100 resulted in a pellet which contained considerable quantities of plastid material. This included RNA polymerase and DNA polymerase activity, much of the DNA, about 30% of the RNA, and 50% of the protein of the washed plastid. The amount of RNA polymerase and DNA polymerase activity associated with the low speed pellet was dependent on the pH during Triton treatment. Significant quantities of the RNA polymerase activity of chloroplasts from spinach, peas, and tobacco were also recovered in the pellet after Triton treatment.
当豌豆质体制备物用 Triton X-100 处理时，膜消失了，色素溶解了，细胞器似乎解体了。用 Triton X-100 处理后，低速离心（2000g）制备物会得到一个沉淀物，其中含有大量质体物质。这包括 RNA 聚合酶和 DNA 聚合酶活性、大部分 DNA、约 30%的 RNA 和 50%的洗涤质体蛋白质。与低速沉淀物相关的 RNA 聚合酶和 DNA 聚合酶活性的量取决于 Triton 处理过程中的 pH 值。在 Triton 处理后，菠菜、豌豆和烟草叶绿体的 RNA 聚合酶活性的大量也被回收在沉淀中。
