Pioneering Research Laboratory, Crops Research Division, Agricultural Research Service, United States Department of Agriculture, Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1970 Jul;46(1):113-7. doi: 10.1104/pp.46.1.113.
Logarithmic growth rates (either fresh or dry weight basis) of tobacco callus tissues grown on 10(-4) to 10(-1) mum cytokinin are increased if gibberellic acid (10(-3)-2 mum) is incorporated into the medium. At higher (1-10 mum) cytokinin concentrations gibberellic acid has little effect on growth rate but extends the duration of logarithmic growth. The gibberellic acid effect is noticeable only after one weight doubling, is dependent on concentration, and occurs when either glucose or sucrose is used as carbon source. The gibberellic acid response includes a decrease in percentage of dry weight relative to control tissues. The maximum dry weight yield, although achieved sooner than controls, does not differ appreciably from yields of tissue not treated with gibberellic acid.
在培养基中添加赤霉素（10（-3）-2 mum）可以提高在 10（-4）至 10（-1） mum 细胞分裂素上生长的烟草愈伤组织的对数生长率（无论是鲜重还是干重基础）。在较高（1-10 mum）细胞分裂素浓度下，赤霉素对生长速率几乎没有影响，但会延长对数生长的持续时间。赤霉素的作用仅在重量增加一倍后才明显，取决于浓度，并且仅在使用葡萄糖或蔗糖作为碳源时才会发生。赤霉素的反应包括相对于对照组织的干重百分比下降。最大干重产量虽然比对照更早达到，但与未用赤霉素处理的组织的产量没有显著差异。
