Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167.
Plant Physiol. 1976 Jun;57(6):894-7. doi: 10.1104/pp.57.6.894.
This paper describes a modified soybean (Glycine max) tissue culture bioassay for cytokinins. Soybean hypocotyls were grown under sterile conditions and sliced into 1-mm sections. Sections were cultured for 5,9,13, or 22 days on a callus medium with zeatin or other cytokinins. The fresh weight of sections increased with the cytokinin concentration from 0.0005 to 1 mum zeatin; 2-fold concentration differences were readily distinguishable at 9 days. The assay should prove to have several advantages over the conventional soybean callus bioassay including convenience, lower variability between tissue samples, and improved resolution. Its specificity is comparable to that of the soybean callus bioassay.
本文描述了一种改良的大豆（Glycine max）组织培养细胞分裂素生物测定法。在无菌条件下培养大豆下胚轴，并切成 1-mm 段。将切片在含有玉米素或其他细胞分裂素的愈伤组织培养基上培养 5、9、13 或 22 天。切片的鲜重随细胞分裂素浓度从 0.0005 至 1 µm 玉米素增加；在 9 天时，2 倍浓度差异很容易区分。与传统的大豆愈伤组织生物测定法相比，该测定法具有几个优点，包括方便、组织样品间变异性低和分辨率提高。其特异性与大豆愈伤组织生物测定法相当。
