一氧化氮生物合成抑制剂和环磷酸鸟苷形成抑制剂对人海绵体平滑肌神经诱发舒张的影响。

The effect of inhibitors of nitric oxide biosynthesis and cyclic GMP formation on nerve-evoked relaxation of human cavernosal smooth muscle.

Pickard R S, Powell P H, Zar M A

Department of Pharmacological Sciences Medical School, Newcastle upon Tyne.

Br J Pharmacol. 1991 Nov;104(3):755-9. doi: 10.1111/j.1476-5381.1991.tb12500.x.

Abstract

The inhibitory transmission in isolated preparations of cavernosal smooth muscle from human penis has been studied. 2. Electrical field stimulation (EFS; 2-64 pulses/train, 0.8 ms pulse duration, 10 Hz) evoked relaxation of preparations treated with guanethidine (50 microM). The EFS-evoked relaxations were atropine-resistant and tetrodotoxin-sensitive indicating their origin to be non-adrenergic, non-cholinergic (NANC) nerve stimulation. 3. EFS-evoked relaxation was attenuated dose-dependently by the nitric oxide (NO)-synthase inhibitor, L-NG-nitro arginine (L-NOARG; 0.3-100 microM) but not by D-NG-nitro arginine. The inhibitory effect of L-NOARG on transmission was antagonized by L-arginine (100 microM), a NO precursor, but not by D-arginine. 4. Incubation with methylene blue (10-50 microM), a known inhibitor of guanylate cyclase activation by NO, caused a concentration-related inhibition of EFS-evoked relaxation. 5. It is concluded that NANC nerve-evoked relaxation of human cavernosal smooth muscle is mediated by NO or a NO-like substance.

摘要

已对来自人类阴茎海绵体平滑肌的离体标本中的抑制性传递进行了研究。2. 电场刺激（EFS；每组2 - 64个脉冲，脉冲持续时间0.8毫秒，频率10赫兹）可引起用胍乙啶（50微摩尔）处理的标本舒张。EFS引起的舒张对阿托品不敏感，对河豚毒素敏感，表明其起源于非肾上腺素能、非胆碱能（NANC）神经刺激。3. EFS引起的舒张被一氧化氮（NO）合酶抑制剂L - NG - 硝基精氨酸（L - NOARG；0.3 - 100微摩尔）剂量依赖性地减弱，但不被D - NG - 硝基精氨酸减弱。L - NOARG对传递的抑制作用被NO前体L - 精氨酸（100微摩尔）拮抗，但不被D - 精氨酸拮抗。4. 用亚甲蓝（10 - 50微摩尔）孵育，亚甲蓝是一种已知的由NO激活鸟苷酸环化酶的抑制剂，可引起EFS引起的舒张的浓度依赖性抑制。5. 得出结论，NANC神经引起的人类海绵体平滑肌舒张是由NO或一种类NO物质介导的。