关于血管活性肠肽并非人海绵体平滑肌舒张性神经递质的证据。
Evidence against vasoactive intestinal polypeptide as the relaxant neurotransmitter in human cavernosal smooth muscle.
作者信息
Pickard R S, Powell P H, Zar M A
机构信息
Department of Pharmacological Sciences, Medical School, University of Newcastle upon Tyne.
出版信息
Br J Pharmacol. 1993 Feb;108(2):497-500. doi: 10.1111/j.1476-5381.1993.tb12831.x.
Abstract
- The putative role of vasoactive intestinal polypeptide (VIP) as the relaxant neurotransmitter in human cavernosal smooth muscle has been studied in isolated tissue preparations. 2. Consistent neurogenic relaxations were evoked by electrical field stimulation (EFS; 2-64 pulses/train, 0.8 ms pulse duration, 10 Hz). VIP (0.1-3 microM) relaxed cavernosal smooth muscle in a dose-dependent fashion. Relaxant responses to both EFS and VIP were reduced in tissue from impotent men. 3. Neurogenic relaxant responses were not diminished in the presence of the VIP-inactivating peptidase, alpha-chymotrypsin (alpha-CT, 2 units ml-1). In contrast VIP-induced relaxations were completely abolished. 4. Inhibition of nitric oxide synthase by NG-nitro-L-arginine (30 microM), and of guanylate cyclase by methylene blue (50 microM) caused highly significant reductions of neurogenic relaxant responses whereas VIP-evoked relaxations were unaffected. 5. It is concluded that VIP-evoked relaxations are not mediated by the NO-guanosine 3':5'-cyclic monophosphate (cyclic GMP) pathway and that VIP release is not essential for neurogenic relaxation of human cavernosal smooth muscle. VIP does not therefore act as the major relaxant neurotransmitter in this tissue.
摘要
- 血管活性肠肽（VIP）作为人海绵体平滑肌舒张性神经递质的假定作用已在离体组织制备中进行了研究。2. 电场刺激（EFS；每串2 - 64个脉冲，脉冲持续时间0.8毫秒，频率10赫兹）可诱发一致的神经源性舒张反应。VIP（0.1 - 3微摩尔）以剂量依赖方式舒张海绵体平滑肌。阳痿男性组织对EFS和VIP的舒张反应均降低。3. 在存在VIP失活肽酶α-糜蛋白酶（α-CT，2单位/毫升）的情况下，神经源性舒张反应并未减弱。相比之下，VIP诱导的舒张反应则完全被消除。4. NG-硝基-L-精氨酸（30微摩尔）抑制一氧化氮合酶，亚甲蓝（50微摩尔）抑制鸟苷酸环化酶，均导致神经源性舒张反应显著降低，而VIP诱发的舒张反应未受影响。5. 得出的结论是，VIP诱发的舒张反应不是由一氧化氮 - 鸟苷3':5'-环磷酸（环磷酸鸟苷）途径介导的，并且VIP释放对于人海绵体平滑肌的神经源性舒张不是必需的。因此，VIP在该组织中并非主要的舒张性神经递质。
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