Plant Sciences Laboratories, Fort Detrick, Frederick, Maryland 21701.
Plant Physiol. 1970 Dec;46(6):790-3. doi: 10.1104/pp.46.6.790.
A 1-hour application of indole-3-acetic acid to bean (Phaseolus vulgaris L. cv. Red Kidney) explants inhibited abscission for an 8-hour aging period. Use of indole-3-acetic acid-(14)C showed that the applied indole-3-acetic acid was conjugated within explant tissue and that this conjugation mechanism accounts for loss of effectiveness of indole-3-acetic acid in inhibiting abscission after 8 hours. Reapplication of indole-3-acetic acid to an explant at a later time, before the induced aging requirement was completed reinhibited abscission. 2,4-Dichlorophenoxyacetic acid, which is not destroyed or conjugated by this system, did not lose its ability to inhibit abscission. It was concluded that indole-3-acetic acid destruction is one of the processes involved in the aging stage of abscission in explants.
1 小时的吲哚乙酸处理可抑制 8 小时老化期的脱落,这种处理可应用于菜豆(Phaseolus vulgaris L. cv. Red Kidney)外植体。使用吲哚乙酸-(14)C 表明,外植体组织内的应用吲哚乙酸发生了结合,这种结合机制解释了吲哚乙酸在 8 小时后抑制脱落的效果丧失。在诱导老化需求完成之前,稍后时间将吲哚乙酸再次应用于外植体,可再次抑制脱落。该系统不会破坏或结合的 2,4-二氯苯氧基乙酸不会失去抑制脱落的能力。结论是,吲哚乙酸的破坏是外植体脱落衰老阶段涉及的过程之一。