Ruesink A W
Department of Botany, Indiana University, Bloomington, Indiana 47401.
Plant Physiol. 1971 Feb;47(2):192-5. doi: 10.1104/pp.47.2.192.
Treatment of living protoplasts from the Avena coleoptile with enzymes and chemicals has provided new information about the external surface of the plasma membrane. Treatments with selected detergents and polyene antibiotics indicate that little sterol is present. The lysis of protoplasts in carboxymethyl-RNase which is enzymatically almost inactive provides strong evidence that the lysis previously observed in RNase is not an indication of RNA in the membrane. Divalent cations inhibit the RNase-induced lysis, indicating that such lysis involves the interaction of RNase with negatively charged sites on the plasma membrane surface. Tyrosinase treatment gives no lysis, showing that tyrosine does not play the role in these plasma membranes attributed to it in some animal cells. Peroxidase does not harm coleoptile protoplasts.
用酶和化学物质处理燕麦胚芽鞘的活原生质体，为质膜外表面提供了新信息。用选定的去污剂和多烯抗生素处理表明几乎不存在甾醇。在酶活性几乎为零的羧甲基核糖核酸酶中，原生质体的裂解有力地证明了先前在核糖核酸酶中观察到的裂解并非膜中存在RNA的迹象。二价阳离子抑制核糖核酸酶诱导的裂解，表明这种裂解涉及核糖核酸酶与质膜表面带负电位点的相互作用。酪氨酸酶处理不会导致裂解，表明酪氨酸在这些质膜中并不发挥在某些动物细胞中所认为的作用。过氧化物酶不会损害胚芽鞘原生质体。
