Science. 1966 Oct 14;154(3746):280-1. doi: 10.1126/science.154.3746.280.
Protoplasts can be readily prepared from higher-plant tissue with a concentrated Myrothecium cellulase preparation. An ionic osmoticum produced a higher yield and more viable protoplasts than did the nonionic man nitol osmoticum. Addition of polyuronidases or of ethylenediaminetetra-acetate did not increase the yield. Protoplasts could be prepared from root, leaf, and callus tissue as well as from coleoptiles.
用浓缩的木霉纤维素酶制剂可以很容易地从高等植物组织中制备原生质体。与非离子甘露醇等渗溶液相比，离子等渗溶液产生的原生质体产量更高、活力更强。添加聚半乳糖醛酸酶或乙二胺四乙酸并没有提高产量。原生质体可以从根、叶和愈伤组织以及胚芽鞘中制备。
