激动素对悬浮培养烟草细胞中h-尿嘧啶和C-亮氨酸掺入的抑制作用。
Kinetin inhibition of h-uracil and C-leucine incorporation by tobacco cells in suspension culture.
作者信息
Nudel U, Bamberger E S
机构信息
Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel.
出版信息
Plant Physiol. 1971 Mar;47(3):400-3. doi: 10.1104/pp.47.3.400.
Abstract
The rate of (14)C-leucine and (3)H-uracil incorporation by tobacco cells (Nicotiana tabaccum var. Samsun N.N.) in suspension culture was simultaneously decreased by the addition of kinetin at concentrations above 2.5 x 10(-5)m. Ribosomal RNA was the first RNA species affected by kinetin. The purine derivatives, adenine and N(6)-methyl-aminopurine, which exhibit low cytokinin activity overcame the inhibitory effects of kinetin. However, purine derivatives without cytokinin activity, guanine, N(6,6)-dimethyl-aminopurine, and 2-aminopurine, did not relieve kinetin inhibition.
摘要
在悬浮培养中，当激动素浓度高于2.5×10⁻⁵m时，烟草细胞（烟草品种Samsun N.N.）对¹⁴C-亮氨酸和³H-尿嘧啶的掺入率同时降低。核糖体RNA是受激动素影响的首个RNA种类。具有低细胞分裂素活性的嘌呤衍生物腺嘌呤和N⁶-甲基氨基嘌呤可克服激动素的抑制作用。然而，没有细胞分裂素活性的嘌呤衍生物鸟嘌呤、N⁶,⁶-二甲基氨基嘌呤和2-氨基嘌呤并不能解除激动素的抑制作用。
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