Shrift A, Ulrich J M
Plant Physiol. 1969 Jun;44(6):893-6. doi: 10.1104/pp.44.6.893.
After incubation for 1 hr with (75)Se-selenate, excised roots of Astragalus crotalariae, a selenium-accumulating species, and A. lentiginosus, a nonaccumulator, had absorbed radioactivity to levels well over the external concentration. About 98% of the radioactivity was ethanol-soluble, and when analyzed by column and paper chromatography and by electrophoresis proved to be selenate. This and previous evidence shows an active transport for selenate. Considerably less radioactivity was absorbed when (75)Se-selenite was supplied to the excised roots, and levels of the ethanol-soluble radioactivity did not exceed the external concentration. A good deal of the radioactivity was ethanol-insoluble. Analysis of the soluble radioactivity from both species showed appreciable conversion of selenite to other forms.
用（75）硒酸盐孵育1小时后，富硒物种猪屎豆黄芪和非富硒物种斑纹黄芪的离体根吸收的放射性水平远高于外部浓度。约98%的放射性物质可溶于乙醇，经柱色谱、纸色谱和电泳分析证明是硒酸盐。这一结果和之前的证据表明存在硒酸盐的主动转运。当向离体根供应（75）亚硒酸盐时，吸收的放射性要少得多，且乙醇可溶性放射性水平未超过外部浓度。大量放射性物质不溶于乙醇。对这两个物种的可溶性放射性物质的分析表明，亚硒酸盐有明显转化为其他形式的情况。
