衰老菜豆茎切片的呼吸与离子转运特性研究
Investigation of respiratory and ion transport properties of aging bean stem slices.
作者信息
Floyd R A, Rains D W
机构信息
Kearney Foundation of Soil Science, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1971 May;47(5):663-7. doi: 10.1104/pp.47.5.663.
Ion and oxygen uptake were studied on aging bean stem slices. Oxygen uptake was high immediately after slicing, decreased to a minimum at 100 minutes, and then increased again. Ion uptake per unit of O(2) uptake data suggested that metabolic energy was utilized almost exclusively for sodium transport in fresh tissue but was diverted to potassium transport as the slices aged. Oxygen and ion uptake in fresh slices was less sensitive to 2,4-dinitrophenol as compared to the aged slices, indicating major metabolic and physiological changes occurred during aging. This was further substantiated by the tissue response to cyanide and antimycin A. Oxygen uptake was decreased by cyanide (22% by 1 mm) and antimycin A (14% by 1 microgram per milliliter) in fresh slices but not in aged slices. Potassium uptake that developed during aging was sensitive to cyanide and antimycin A. The results are pertinent to understanding the role of the stem in regulating ion transport in plants.
对老化的菜豆茎切片进行了离子吸收和氧气吸收的研究。切片后氧气吸收量立即很高，在100分钟时降至最低，然后又再次升高。每单位氧气吸收量的离子吸收数据表明，代谢能量在新鲜组织中几乎完全用于钠的运输，但随着切片老化，转而用于钾的运输。与老化切片相比，新鲜切片中的氧气和离子吸收对2,4-二硝基苯酚的敏感性较低，这表明老化过程中发生了主要的代谢和生理变化。组织对氰化物和抗霉素A的反应进一步证实了这一点。新鲜切片中的氧气吸收被氰化物（1 mM时降低22%）和抗霉素A（每毫升1微克时降低14%）所抑制，但老化切片中则不然。老化过程中出现的钾吸收对氰化物和抗霉素A敏感。这些结果有助于理解茎在调节植物离子运输中的作用。
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