Johnston S L
Department of Laboratory Medicine, Bellin Memorial Hospital, Green Bay, Wisconsin 54305.
Diagn Microbiol Infect Dis. 1991 Sep-Oct;14(5):373-5. doi: 10.1016/0732-8893(91)90063-l.
The performance of locally produced human rhabdomyosarcoma (RD) cells was shown to be superior to human foreskin fibroblast (SF) and HEp-2 cells for the isolation of the herpes simplex virus (HSV) from clinical specimens. A comparison of sensitivity, clarity of cytophathic effect, and ease of growing the cells for the isolation of HSV was made on these cell lines. A total of 1351 fresh clinical specimens, submitted for the isolation of HSV, were inoculated into RD, HEp-2, and SF cell culture tubes. The cultures were incubated for 7 days at 35 degrees C on a roller drum and observed daily for cytophathic effect (CPE). Of these specimens, 318 (23.5%) were positive for HSV. In 287 (90%) of these positive specimens, the RD cell line was the first, either alone or simultaneously with another cell line, to display typical HSV CPE. This CPE was clearly recognized by the appearance of clusters of rounded cells and associated multinucleated giant cells. In 241 (76%) of the positive specimens, the RD cell line displayed CPE on the first or second day of incubation. These results demonstrate that the RD cell line was more rapid than the SF and HEp-2 cell lines for the detection of HSV and suggests that RD cells should be included in a tissue culture program designed for the detection of HSV.
结果表明，就从临床标本中分离单纯疱疹病毒（HSV）而言，本地生产的人横纹肌肉瘤（RD）细胞的表现优于人包皮成纤维细胞（SF）和HEp-2细胞。对这些细胞系在HSV分离方面的敏感性、细胞病变效应的清晰度以及细胞生长的难易程度进行了比较。总共1351份提交用于HSV分离的新鲜临床标本被接种到RD、HEp-2和SF细胞培养管中。培养物在35摄氏度下于滚筒上孵育7天，并每天观察细胞病变效应（CPE）。在这些标本中，318份（23.5%）HSV呈阳性。在这些阳性标本中的287份（90%）中，RD细胞系是第一个单独或与另一个细胞系同时显示典型HSV CPE的。通过圆形细胞簇和相关的多核巨细胞的出现可清楚识别这种CPE。在241份（76%）阳性标本中，RD细胞系在孵育的第一天或第二天就显示出CPE。这些结果表明，RD细胞系在检测HSV方面比SF和HEp-2细胞系更快，并表明在设计用于检测HSV的组织培养方案中应纳入RD细胞。
