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1
Rapid isolation of herpes simplex virus by using mink lung and rhabdomyosarcoma cell cultures.利用貂肺和横纹肌肉瘤细胞培养快速分离单纯疱疹病毒。
J Clin Microbiol. 1990 Dec;28(12):2806-7. doi: 10.1128/jcm.28.12.2806-2807.1990.
2
Comparison of shell vials and conventional tubes seeded with rhabdomyosarcoma and MRC-5 cells for the rapid detection of herpes simplex virus.用于快速检测单纯疱疹病毒的接种横纹肌肉瘤和MRC-5细胞的空斑试验瓶与传统试管的比较
J Clin Microbiol. 1991 Dec;29(12):2701-3. doi: 10.1128/jcm.29.12.2701-2703.1991.
3
Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining.通过离心和免疫过氧化物酶染色快速检测临床标本中的单纯疱疹病毒。
J Clin Microbiol. 1986 Apr;23(4):683-6. doi: 10.1128/jcm.23.4.683-686.1986.
4
Comparison of enzyme immunoassay, shell vial culture, and conventional cell culture for the rapid detection of herpes simplex virus.酶免疫测定、空斑小室培养和传统细胞培养用于快速检测单纯疱疹病毒的比较
Diagn Microbiol Infect Dis. 1990 May-Jun;13(3):241-4. doi: 10.1016/0732-8893(90)90066-5.
5
Comparison of human rhabdomyosarcoma, HEp-2, and human foreskin fibroblast cells for the isolation of herpes simplex virus from clinical specimens.用于从临床标本中分离单纯疱疹病毒的人横纹肌肉瘤、HEp-2细胞和人包皮成纤维细胞的比较
Diagn Microbiol Infect Dis. 1991 Sep-Oct;14(5):373-5. doi: 10.1016/0732-8893(91)90063-l.
6
More rapid isolation of herpes simplex virus in a continuous line of mink lung cells than in Vero or human fibroblast cells.在貂肺细胞连续系中单纯疱疹病毒的分离速度比在非洲绿猴肾细胞或人成纤维细胞中更快。
Diagn Microbiol Infect Dis. 1984 Sep;2(4):317-24. doi: 10.1016/0732-8893(84)90063-4.
7
Comparison of cell culture with an immunoperoxidase kit for rapid diagnosis of herpes simplex virus infections.用于单纯疱疹病毒感染快速诊断的免疫过氧化物酶试剂盒与细胞培养的比较。
Diagn Microbiol Infect Dis. 1984 Sep;2(4):343-5. doi: 10.1016/0732-8893(84)90067-1.
8
Performance of six cell lines in the suspension-infection test used for the detection of herpes simplex virus.用于检测单纯疱疹病毒的悬浮感染试验中六种细胞系的表现
Diagn Microbiol Infect Dis. 1998 Oct;32(2):81-4. doi: 10.1016/s0732-8893(98)00071-6.
9
Rapid detection of herpes simplex virus in culture by in situ hybridization.通过原位杂交技术在培养物中快速检测单纯疱疹病毒。
J Virol Methods. 1993 Jan;41(1):1-7. doi: 10.1016/0166-0934(93)90157-m.
10
Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.细胞培养敏感性和病毒浓度对通过细胞病变效应和免疫过氧化物酶染色快速检测单纯疱疹病毒的影响。
J Clin Microbiol. 1987 Aug;25(8):1401-5. doi: 10.1128/jcm.25.8.1401-1405.1987.

引用本文的文献

1
The laboratory diagnosis of herpes simplex virus infections.单纯疱疹病毒感染的实验室诊断。
Can J Infect Dis Med Microbiol. 2005 Mar;16(2):92-8. doi: 10.1155/2005/318294.
2
Comparison of shell vials and conventional tubes seeded with rhabdomyosarcoma and MRC-5 cells for the rapid detection of herpes simplex virus.用于快速检测单纯疱疹病毒的接种横纹肌肉瘤和MRC-5细胞的空斑试验瓶与传统试管的比较
J Clin Microbiol. 1991 Dec;29(12):2701-3. doi: 10.1128/jcm.29.12.2701-2703.1991.
3
Comparative evaluation of four commercially available monoclonal antibodies for culture confirmation of herpes simplex virus infection.四种市售单克隆抗体用于单纯疱疹病毒感染培养确认的比较评估。
J Clin Microbiol. 1992 Jul;30(7):1874-5. doi: 10.1128/jcm.30.7.1874-1875.1992.

本文引用的文献

1
Comparison of human fibroblast cells and primary rabbit kidney cells for isolation of herpes simplex virus.用于单纯疱疹病毒分离的人成纤维细胞与原代兔肾细胞的比较。
J Clin Microbiol. 1984 Apr;19(4):548-9. doi: 10.1128/jcm.19.4.548-549.1984.
2
More rapid isolation of herpes simplex virus in a continuous line of mink lung cells than in Vero or human fibroblast cells.在貂肺细胞连续系中单纯疱疹病毒的分离速度比在非洲绿猴肾细胞或人成纤维细胞中更快。
Diagn Microbiol Infect Dis. 1984 Sep;2(4):317-24. doi: 10.1016/0732-8893(84)90063-4.
3
Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.细胞培养敏感性和病毒浓度对通过细胞病变效应和免疫过氧化物酶染色快速检测单纯疱疹病毒的影响。
J Clin Microbiol. 1987 Aug;25(8):1401-5. doi: 10.1128/jcm.25.8.1401-1405.1987.
4
Evaluation of a rapid latex slide agglutination test for herpes simplex virus as a specimen screen and culture identification method.评价一种用于单纯疱疹病毒的快速乳胶玻片凝集试验作为标本筛查和培养鉴定方法的效果。
J Clin Microbiol. 1987 May;25(5):936-7. doi: 10.1128/jcm.25.5.936-937.1987.
5
Comparison of the Ortho Cultureset (Vero cells) and Difco Cellmatics system (mink lung and primary rabbit kidney cells) for the detection of herpes simplex virus from clinical specimens.用于从临床标本中检测单纯疱疹病毒的Ortho Cultureset（Vero细胞）和Difco Cellmatics系统（貂肺和原代兔肾细胞）的比较。
Diagn Microbiol Infect Dis. 1987 Feb;6(2):145-9. doi: 10.1016/0732-8893(87)90099-x.
6
Evaluation of five cell types for the isolation of herpes simplex virus.用于分离单纯疱疹病毒的五种细胞类型的评估
Diagn Microbiol Infect Dis. 1986 Jul;5(2):127-33. doi: 10.1016/0732-8893(86)90114-8.
7
Comparison of commercial sources of primary rabbit kidney and MRC-5 cell cultures for herpes simplex virus isolation.用于单纯疱疹病毒分离的原代兔肾细胞和MRC-5细胞培养物的商业来源比较。
Diagn Microbiol Infect Dis. 1986 Feb;4(2):147-52. doi: 10.1016/0732-8893(86)90149-5.
8
Comparison of diploid fibroblast and rabbit kidney tissue cultures and a diploid fibroblast microtiter plate system for the isolation of herpes simplex virus.用于分离单纯疱疹病毒的二倍体成纤维细胞和兔肾组织培养物以及二倍体成纤维细胞微量滴定板系统的比较
J Clin Microbiol. 1988 Sep;26(9):1772-4. doi: 10.1128/jcm.26.9.1772-1774.1988.
9
Detection of herpes simplex virus in conventional tube cell cultures and in shell vials with a DNA probe kit and monoclonal antibodies.使用DNA探针试剂盒和单克隆抗体在传统试管细胞培养物和空斑试验瓶中检测单纯疱疹病毒。
J Clin Microbiol. 1988 Jan;26(1):22-4. doi: 10.1128/jcm.26.1.22-24.1988.
10
Determination of cell line suitability for rapid isolation of herpes simplex virus.确定细胞系对单纯疱疹病毒快速分离的适用性。
J Virol Methods. 1987 Oct;18(1):37-46. doi: 10.1016/0166-0934(87)90108-x.

利用貂肺和横纹肌肉瘤细胞培养快速分离单纯疱疹病毒。

Rapid isolation of herpes simplex virus by using mink lung and rhabdomyosarcoma cell cultures.

作者信息

Johnston S L, Wellens K, Siegel C S

机构信息

Department of Laboratory Medicine, Bellin Memorial Hospital, Green Bay, Wisconsin 54305.

出版信息

J Clin Microbiol. 1990 Dec;28(12):2806-7. doi: 10.1128/jcm.28.12.2806-2807.1990.

DOI:10.1128/jcm.28.12.2806-2807.1990
PMID:2177754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268279/
Abstract

Highly sensitive and rapid results can be obtained by isolating herpes simplex virus from clinical specimens in simple cell culture with rhabdomyosarcoma (RD) cells. In this study, 3,186 clinical specimens were inoculated into locally produced, equivalent-age RD and mink lung (ML) cells. Of 727 positive isolates, all (100%) were isolated from RD cells and only 691 (95%) were isolated from ML cells. Furthermore, 162 of the positive isolates (22%) were isolated in RD cells earlier than in ML cells. RD cells are continuous and can be cultivated in house without decreasing sensitivity as the passage number increases. They produce a highly distinguishable cytopathic effect in response to herpes simplex virus and maintain intense confirmatory staining patterns.

摘要

通过在含有横纹肌肉瘤（RD）细胞的简单细胞培养中从临床标本中分离单纯疱疹病毒，可获得高度敏感且快速的结果。在本研究中，将3186份临床标本接种到本地生产的、年龄相当的RD细胞和貂肺（ML）细胞中。在727份阳性分离株中，所有（100%）均从RD细胞中分离得到，而仅691份（95%）从ML细胞中分离得到。此外，162份阳性分离株（22%）在RD细胞中的分离时间早于在ML细胞中。RD细胞是连续的，可在室内培养，且不会随着传代次数增加而降低敏感性。它们在受到单纯疱疹病毒感染时会产生高度可辨别的细胞病变效应，并保持强烈的确诊染色模式。