利用貂肺和横纹肌肉瘤细胞培养快速分离单纯疱疹病毒。
Rapid isolation of herpes simplex virus by using mink lung and rhabdomyosarcoma cell cultures.
作者信息
Johnston S L, Wellens K, Siegel C S
机构信息
Department of Laboratory Medicine, Bellin Memorial Hospital, Green Bay, Wisconsin 54305.
出版信息
J Clin Microbiol. 1990 Dec;28(12):2806-7. doi: 10.1128/jcm.28.12.2806-2807.1990.
Abstract
Highly sensitive and rapid results can be obtained by isolating herpes simplex virus from clinical specimens in simple cell culture with rhabdomyosarcoma (RD) cells. In this study, 3,186 clinical specimens were inoculated into locally produced, equivalent-age RD and mink lung (ML) cells. Of 727 positive isolates, all (100%) were isolated from RD cells and only 691 (95%) were isolated from ML cells. Furthermore, 162 of the positive isolates (22%) were isolated in RD cells earlier than in ML cells. RD cells are continuous and can be cultivated in house without decreasing sensitivity as the passage number increases. They produce a highly distinguishable cytopathic effect in response to herpes simplex virus and maintain intense confirmatory staining patterns.
摘要
通过在含有横纹肌肉瘤（RD）细胞的简单细胞培养中从临床标本中分离单纯疱疹病毒，可获得高度敏感且快速的结果。在本研究中，将3186份临床标本接种到本地生产的、年龄相当的RD细胞和貂肺（ML）细胞中。在727份阳性分离株中，所有（100%）均从RD细胞中分离得到，而仅691份（95%）从ML细胞中分离得到。此外，162份阳性分离株（22%）在RD细胞中的分离时间早于在ML细胞中。RD细胞是连续的，可在室内培养，且不会随着传代次数增加而降低敏感性。它们在受到单纯疱疹病毒感染时会产生高度可辨别的细胞病变效应，并保持强烈的确诊染色模式。
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