Walton D C, Sondheimer E
Department of Chemistry, Plant Physiology-Biochemistry Group, SUNY College of Forestry at Syracuse University, Syracuse, New York 13210.
Plant Physiol. 1972 Mar;49(3):285-9. doi: 10.1104/pp.49.3.285.
Excised embryonic bean axes (Phaseolus vulgaris, var. White Marrowfat) rapidly metabolize 2-(14)C-(+/-)-abscisic acid to two compounds, M-1 and M-2, which have very low growth-inhibitory activity. Chemical tests indicate the M-1 and M-2 are not previously described abscisic acid metabolites. M-2 accumulates in the axes and evidence is presented for the hypothesis that abscisic acid --> M-1 --> M-2. Zeatin, which partially reverses the abscisic acid-mediated growth inhibition of axes, neither decreases abscisic acid uptake nor causes any major changes in its metabolism. It was observed that axes transferred from abscisic acid-containing solutions to buffer resume control rates of fresh weight increase while still containing considerable quantities of abscisic acid.
切除的菜豆胚轴（菜豆，白髓变种）能迅速将2-(14)C-(+/-)-脱落酸代谢为两种化合物，M-1和M-2，它们的生长抑制活性非常低。化学测试表明，M-1和M-2并非先前描述的脱落酸代谢产物。M-2在胚轴中积累，并且有证据支持脱落酸→M-1→M-2这一假说。玉米素能部分逆转脱落酸介导的胚轴生长抑制作用，但既不降低脱落酸的摄取量，也不会使其代谢发生任何重大变化。据观察，从含脱落酸的溶液转移到缓冲液中的胚轴，在仍含有相当数量脱落酸的情况下，恢复了鲜重增加的对照速率。
