叶绿体和细胞质酶：IV. 豌豆叶果糖1,6 -二磷酸醛缩酶
Chloroplast and Cytoplasmic Enzymes: IV. Pea Leaf Fructose 1,6-Diphosphate Aldolases.
作者信息
Anderson L E, Pacold I
机构信息
Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680.
出版信息
Plant Physiol. 1972 Mar;49(3):393-7. doi: 10.1104/pp.49.3.393.
Abstract
Several peaks of aldolase activity are found in the isoelectric focusing pattern of pea (Pisum sativum) leaf chloroplast extracts. One peak, separated by 0.5 pH unit from the major chloroplast aldolase peak, is found when cytoplasmic extracts are focused. The chloroplast and cytoplasmic enzymes have a pH 7.4 optimum with fructose 1,6-diphosphate. The Michaelis constant for fructose-1,6-diphosphate is 19 muM for the chloroplast, 21 muM for the cytoplasmic enzyme, and for sedoheptulose 1,7-diphosphate, 8 muM for the chloroplast enzyme, 18 muM for the cytoplasmic enzyme. Both enzymes are inhibited by d-glyceraldehyde 3-phosphate and by ribulose 1,5-diphosphate. The similarity in the catalytic properties of the isoenzymes suggests that both enzymes have an amphibolic role in carbon metabolism in the green leaf.
摘要
在豌豆（Pisum sativum）叶片叶绿体提取物的等电聚焦图谱中发现了几个醛缩酶活性峰。当对细胞质提取物进行聚焦时，发现有一个峰与主要的叶绿体醛缩酶峰相隔0.5个pH单位。叶绿体和细胞质中的酶在以1,6-二磷酸果糖为底物时，最适pH为7.4。叶绿体醛缩酶对1,6-二磷酸果糖的米氏常数为19μM，细胞质醛缩酶为21μM；对于1,7-二磷酸景天庚酮糖，叶绿体醛缩酶的米氏常数为8μM，细胞质醛缩酶为18μM。两种酶都受到3-磷酸d-甘油醛和1,5-二磷酸核酮糖的抑制。同工酶催化特性的相似性表明，这两种酶在绿叶的碳代谢中都具有兼性作用。
相似文献
1
Chloroplast and Cytoplasmic Enzymes: IV. Pea Leaf Fructose 1,6-Diphosphate Aldolases.叶绿体和细胞质酶：IV. 豌豆叶果糖1,6 -二磷酸醛缩酶
Plant Physiol. 1972 Mar;49(3):393-7. doi: 10.1104/pp.49.3.393.
2
Chloroplast and cytoplasmic enzymes: three distinct isoenzymes associated with the reductive pentose phosphate cycle.叶绿体和细胞质酶：与还原性戊糖磷酸循环相关的三种不同同工酶。
Plant Physiol. 1970 May;45(5):583-5. doi: 10.1104/pp.45.5.583.
3
Distinction between Cytosol and Chloroplast Fructose-Bisphosphate Aldolases from Pea, Wheat, and Corn Leaves.豌豆、小麦和玉米叶片细胞质和叶绿体果糖-1,6-二磷酸醛缩酶的区别。
Plant Physiol. 1986 Feb;80(2):301-4. doi: 10.1104/pp.80.2.301.
4
Appearance of Three Chloroplast Isoenzymes in Dark-grown Pea Plants and Pea Seeds.黑暗中生长的豌豆植株和豌豆种子中三种叶绿体同工酶的出现。
Plant Physiol. 1973 Feb;51(2):259-62. doi: 10.1104/pp.51.2.259.
5
D-glyceraldehyde 3-phosphate dehydrogenases of higher plants.高等植物的D-甘油醛-3-磷酸脱氢酶
Plant Physiol. 1968 Nov;43(11):1805-12. doi: 10.1104/pp.43.11.1805.
6
Chloroplast and cytoplasmic enzymes : V. Pea-leaf carbonic anhydrases.叶绿体和细胞质酶：V. 豌豆叶碳酸酐酶。
Planta. 1974 Sep;118(3):235-40. doi: 10.1007/BF00384779.
7
Chloroplast and cytoplasmic enzymes: isolation and sequencing of cDNAs coding for two distinct pea chloroplast aldolases.叶绿体和细胞质酶：编码两种不同豌豆叶绿体醛缩酶的cDNA的分离与测序
Arch Biochem Biophys. 1992 Oct;298(1):192-7. doi: 10.1016/0003-9861(92)90112-a.
8
Isolation and characterization of the cytosolic and chloroplast forms of spinach leaf fructose diphosphate aldolase.菠菜叶果糖二磷酸醛缩酶胞质和叶绿体形式的分离与鉴定
J Biol Chem. 1984 Jan 25;259(2):1011-7.
9
Chloroplast aldolase is controlled by a nuclear gene.叶绿体醛缩酶受一个核基因控制。
Plant Physiol. 1970 Dec;46(6):819-20. doi: 10.1104/pp.46.6.819.
10
Comparative Enzymology of the Glyceraldehyde 3-Phosphate Dehydrogenases from Pisum sativum.豌豆甘油醛-3-磷酸脱氢酶的比较酶学研究。
Plant Physiol. 1974 Sep;54(3):312-9. doi: 10.1104/pp.54.3.312.
引用本文的文献
1
Chloroplast and cytoplasmic enzymes : V. Pea-leaf carbonic anhydrases.叶绿体和细胞质酶：V. 豌豆叶碳酸酐酶。
Planta. 1974 Sep;118(3):235-40. doi: 10.1007/BF00384779.
2
The effect of pyridoxal phosphate on the activity of aldolase from Lemna minor L.磷酸吡哆醛对浮萍（ Lemna minor L. ）醛缩酶活性的影响。
Planta. 1977 Jan;137(3):265-70. doi: 10.1007/BF00388161.
3
Development of cytosol and chloroplast aldolases during germination of spinach seeds.叶绿体和细胞质醛缩酶在菠菜种子萌发过程中的发育。
Planta. 1985 May;164(1):109-14. doi: 10.1007/BF00391034.
4
α-1,4-glucan phosphorylase forms from leaves of spinach (Spinacia oleracea L.) : II. Peptide patterns and immunological properties. A comparison with other phosphorylase forms.α-1,4-葡聚糖磷酸化酶在菠菜（Spinacia oleracea L.）叶片中的形成：Ⅱ. 肽图谱和免疫学特性。与其他磷酸化酶形式的比较。
Planta. 1986 Jun;168(2):222-31. doi: 10.1007/BF00402967.
5
Structural Similarities between Spinach Chloroplast and Cytosolic Class I Fructose 1,6-Bisphosphate Aldolases : Immunochemical and Amino-Terminal Amino Acid Sequence Analysis.菠菜叶绿体与胞质溶胶类果糖 1,6-二磷酸醛缩酶的结构相似性：免疫化学和氨基末端氨基酸序列分析。
Plant Physiol. 1989 Dec;91(4):1393-401. doi: 10.1104/pp.91.4.1393.
6
Distinction between Cytosol and Chloroplast Fructose-Bisphosphate Aldolases from Pea, Wheat, and Corn Leaves.豌豆、小麦和玉米叶片细胞质和叶绿体果糖-1,6-二磷酸醛缩酶的区别。
Plant Physiol. 1986 Feb;80(2):301-4. doi: 10.1104/pp.80.2.301.
7
Light-Induced Release of Bound Glucose-6-phosphate Dehydrogenase to the Stroma in Pea Chloroplasts.光诱导豌豆叶绿体中结合态葡萄糖-6-磷酸脱氢酶释放至基质
Plant Physiol. 1981 Aug;68(2):279-83. doi: 10.1104/pp.68.2.279.
8
Isolation of cytoplasmic enzymes from pollen.从花粉中分离细胞质酶。
Plant Physiol. 1980 Sep;66(3):400-3. doi: 10.1104/pp.66.3.400.
9
Characterization and kinetics of isoenzymes of pyruvate kinase from developing castor bean endosperm.蓖麻籽发育胚乳中丙酮酸激酶同工酶的特性及动力学
Plant Physiol. 1980 Jun;65(6):1188-93. doi: 10.1104/pp.65.6.1188.
10
Modulation of Chloroplast Fructose-1,6-bisphosphatase Activity by Light.光对叶绿体果糖-1,6-二磷酸酶活性的调节
Plant Physiol. 1979 Sep;64(3):491-4. doi: 10.1104/pp.64.3.491.
本文引用的文献
1
Chloroplast and cytoplasmic enzymes: three distinct isoenzymes associated with the reductive pentose phosphate cycle.叶绿体和细胞质酶：与还原性戊糖磷酸循环相关的三种不同同工酶。
Plant Physiol. 1970 May;45(5):583-5. doi: 10.1104/pp.45.5.583.
2
Role of Aldolase in Photosynthesis. II Demonstration of Aldolase Types in Photosynthetic Organisms.醛缩酶在光合作用中的作用。II. 光合生物中醛缩酶类型的证明。
Plant Physiol. 1968 May;43(5):793-8. doi: 10.1104/pp.43.5.793.
3
The preparation of sedoheptulose diphosphate.
J Biol Chem. 1956 Feb;218(2):745-52.
4
Purification and properties of fructose diphosphate aldolase from spinach leaves.菠菜叶中果糖二磷酸醛缩酶的纯化及性质
Eur J Biochem. 1967 Mar;1(1):117-24. doi: 10.1007/978-3-662-25813-2_19.
5
Isolation and characterization of fructose diphosphate aldolase from Pisum sativum.
Biochim Biophys Acta. 1967 Jul 11;139(2):460-8. doi: 10.1016/0005-2744(67)90049-6.
6
The subunit structure of the fructose diphosphate aldolase from spinach leaf.菠菜叶中果糖二磷酸醛缩酶的亚基结构。
Arch Biochem Biophys. 1969 Jun;132(1):286-93. doi: 10.1016/0003-9861(69)90364-6.
7
Photosynthesis in Rhodospirillum rubrum. IV. Isolation and characterization of ribulose 1,5-diphosphate carboxylase.深红红螺菌中的光合作用。IV. 1,5-二磷酸核酮糖羧化酶的分离与特性
J Biol Chem. 1969 Jun 25;244(12):3105-9.
8
Labeling of the active site of aldolase with glyceraldehyde 3-phosphate and erythrose 4-phosphate.用3-磷酸甘油醛和4-磷酸赤藓糖对醛缩酶活性位点进行标记。
Biochem Biophys Res Commun. 1968 Mar 27;30(6):665-72. doi: 10.1016/0006-291x(68)90564-0.
9
Isolation of crystalline pig kidney aldolase B.
Biochim Biophys Acta. 1971 Feb 10;227(2):460-3. doi: 10.1016/0005-2744(71)90077-5.
10
Studies on the substrates of D-fructose 1,6-diphosphate aldolase in solution.
Biochemistry. 1970 Jun 9;9(12):2454-62. doi: 10.1021/bi00814a010.
Zotero 插件