Anderson L E, Chin H M, Gupta V K
Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680.
Plant Physiol. 1979 Sep;64(3):491-4. doi: 10.1104/pp.64.3.491.
Inhibitor experiments indicate that light effect mediator(II) which is reductively activated by transfer of electrons from the photosynthetic electron transport system at or beyond ferredoxin, is involved in activation by light of fructose-1,6-bisphosphatase in the pea plant. Activation proceeds optimally when the pH is low and Mg(2+) is 10 millimolar. Modulation by light results in increases in maximal velocity, apparently as a result of changes in enzyme conformation. Pea leaf thylakoids are effective in modulating the activity of glyceraldehyde-3-phosphate dehydrogenase but not of fructose-1,6-bisphosphatase or glucose-6-phosphate dehydrogenase in Kalanchoë stromal extracts. There is apparently species specificity for modulation of some, but not all, of the modulatable enzymes.
抑制剂实验表明,光效应介质(II)通过在铁氧化还原蛋白或其之后从光合电子传递系统转移电子而被还原激活,参与豌豆植株中果糖-1,6-二磷酸酶的光激活过程。当pH值较低且Mg(2+)为10毫摩尔时,激活作用最佳。光调节导致最大反应速度增加,这显然是酶构象变化的结果。豌豆叶类囊体对景天属基质提取物中甘油醛-3-磷酸脱氢酶的活性有调节作用,但对果糖-1,6-二磷酸酶或葡萄糖-6-磷酸脱氢酶没有调节作用。对于一些但不是所有可调节酶的调节,显然存在物种特异性。