Leonard R T, Hanson J B
Department of Botany, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1972 Mar;49(3):430-5. doi: 10.1104/pp.49.3.430.
Washing excised or intact primary roots of corn (Zea mays L., WF9 x M14) in aerated distilled water or dilute salt solutions for 2 hours induced doubling of the rate of accumulation of various nutrient ions and solutes. This response to washing depended upon aerobic metabolism, but involved no increase in aerobic respiration. Excision of root tissue was not required as the effect could be obtained with intact root systems. Increased phosphate absorption followed after a lag period of 30 to 40 minutes and continued for 6 hours before leveling off at about 3.5 times the initial rate. Chloramphenicol was not inhibitory to the development of increased absorption, while inhibitors of RNA and protein synthesis were. Auxins and kinetin were also inhibitory, but so was the antiauxin, 2,4,6-trichlorophenoxyacetic acid.Kinetic analyses of phosphate and potassium ((86)Rb) absorption showed no change in apparent Michaelis constant. Electron microscopic examination and analysis for membrane protein and lipid-phosphate content after washing showed no proliferation of membrane or other changes in cell structure and composition. It appears that washing augments or activates existing membrane transport mechanisms by processes involving protein synthesis.
将玉米（Zea mays L.，WF9×M14）切除的或完整的初生根在通气的蒸馏水中或稀盐溶液中洗涤2小时，会使各种营养离子和溶质的积累速率加倍。这种对洗涤的反应依赖于有氧代谢，但有氧呼吸并未增加。不需要切除根组织，因为完整的根系也能获得这种效果。磷酸盐吸收增加在30至40分钟的滞后时间后出现，并持续6小时，然后在约为初始速率3.5倍时趋于平稳。氯霉素对吸收增加的发展没有抑制作用，而RNA和蛋白质合成抑制剂则有抑制作用。生长素和激动素也有抑制作用，但抗生长素2,4,6-三氯苯氧乙酸也有抑制作用。对磷酸盐和钾（（86）Rb）吸收的动力学分析表明，表观米氏常数没有变化。洗涤后对膜蛋白和脂质磷酸盐含量进行的电子显微镜检查和分析显示，膜没有增殖，细胞结构和组成也没有其他变化。看来洗涤通过涉及蛋白质合成的过程增强或激活了现有的膜转运机制。
