Department of Biochemistry, University of Aberdeen, and Department of Plant Physiology, The Macaulay Institute for Soil Research, Aberdeen, Scotland.
Plant Physiol. 1970 Aug;46(2):227-32. doi: 10.1104/pp.46.2.227.
Although cycloheximide is extremely inhibitory to protein synthesis in vivo in higher plants, the reported insensitivity of some plant ribosomes suggests that it may not invariably act at the ribosomal level. This suggestion is reinforced by results obtained with red beet storage tissue disks, the respiration of which is stimulated by cycloheximide at 1 microgram per milliliter. Inorganic ion uptake by these disks is inhibited by cycloheximide at 1 microgram per milliliter while the uptake of organic compounds, by comparison, is unaffected. Ion uptake by all nongreen tissues tested is inhibited by cycloheximide, but leaf tissue is unaffected, indicating that the ion absorption mechanism in the leaf may differ fundamentally from that in the root. It is concluded that cycloheximide can affect cellular metabolism other than by inhibiting protein synthesis and that the inhibition of ion uptake may be due to disruption of the energy supply.
尽管环己亚胺在体内对高等植物的蛋白质合成有极强的抑制作用，但有些植物核糖体对此不敏感的报道表明，它可能并不总是在核糖体水平上起作用。这一观点得到了以下结果的支持：用红色甜菜贮藏组织薄片进行的实验表明，环己亚胺在 1 微克/毫升时可刺激其呼吸。环己亚胺在 1 微克/毫升时能抑制这些薄片对无机离子的摄取，而相比之下，对有机化合物的摄取则不受影响。环己亚胺能抑制所有非绿色组织的离子摄取，但叶组织不受影响，这表明叶组织的离子吸收机制可能与根组织根本不同。因此可以得出结论，环己亚胺除了通过抑制蛋白质合成以外，还能影响细胞代谢，而离子摄取的抑制可能是由于能量供应的中断。
