Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.
Plant Physiol. 1979 Jan;63(1):35-41. doi: 10.1104/pp.63.1.35.
The distribution of cytokinin activity in wheat (Triticum aestivum) germ tRNA fractionated by BD-cellulose and RPC-5 chromatography has been examined. As in other organisms, the cytokinin moieties in wheat germ tRNA appear to be restricted to tRNA species that would be expected to respond to codons beginning with U. Only a few of the wheat germ tRNA species in this coding group actually contain cytokinin modifications. Cytokinin activity was associated with isoaccepting tRNA(Ser) species and with a minor tRNA(Leu) species from wheat germ. All other wheat germ tRNA species corresponding to codons beginning with U were devoid of cytokinin activity in the tobacco callus bioassay.
用 BD-纤维素和 RPC-5 层析法分离的小麦胚 tRNA 中细胞分裂素活性的分布已被检测。与其他生物体一样，小麦胚 tRNA 中的细胞分裂素部分似乎仅限于那些预期能响应以 U 开头的密码子的 tRNA 种类。在这个编码组中，只有少数几种小麦胚 tRNA 实际上含有细胞分裂素修饰。细胞分裂素活性与同工的 tRNA(Ser)种类以及来自小麦胚的少量 tRNA(Leu)种类有关。烟草愈伤组织生物测定中，所有其他对应于以 U 开头的密码子的小麦胚 tRNA 种类均不含细胞分裂素活性。
