Meudt W J, Stecher K J
Plant Science Research Division, Agricultural Research Service, United States Department of Agriculture, Plant Industry Station, Beltsville, Maryland 20705.
Plant Physiol. 1972 Jul;50(1):157-60. doi: 10.1104/pp.50.1.157.
Peroxidase catalyzes the oxidation of indole-3-acetic acid. The primary products of this reaction stimulate growth in plants. Therefore, our concept is that an increase in peroxidase activity will increase the effect of indole-3-acetic acid as a growth hormone. Our objective was to study the effect of 2,3,5-triiodobenzoic acid, a growth regulator, on isoperoxidases in the cell wall and cytoplasm of Nicotiana. Isoperoxidases from the cell wall and cytoplasmic fractions were separated by acrylamide gel electrophoresis. We found that 2,3,5-triiodobenzoic acid and indole-3-acetic acid increase peroxidase activity in the cell wall. Since both 2,3,5-triiodobenzoic acid and indole-3-acetic acid increase the activity of the same isoperoxidase, we conclude that 2,3,5-triiodobenzoic acid synergizes rather than antagonizes auxin action, and we suggest that this increase in indole-3-acetic acid oxidase activity sensitizes plant tissues to auxin.
过氧化物酶催化吲哚 - 3 - 乙酸的氧化反应。该反应的主要产物能促进植物生长。因此，我们的观点是过氧化物酶活性的增加会增强吲哚 - 3 - 乙酸作为生长激素的作用效果。我们的目的是研究生长调节剂2,3,5 - 三碘苯甲酸对烟草细胞壁和细胞质中同功过氧化物酶的影响。通过丙烯酰胺凝胶电泳分离细胞壁和细胞质组分中的同功过氧化物酶。我们发现2,3,5 - 三碘苯甲酸和吲哚 - 3 - 乙酸能提高细胞壁中的过氧化物酶活性。由于2,3,5 - 三碘苯甲酸和吲哚 - 3 - 乙酸均能提高同一种同功过氧化物酶的活性，我们得出结论：2,3,5 - 三碘苯甲酸增强而非拮抗生长素的作用，并且我们认为吲哚 - 3 - 乙酸氧化酶活性的这种增加使植物组织对生长素更敏感。
