Department of Plant Biology, University of Murcia, Campus of Espinardo, E-30071, Murcia, Spain.
Planta. 1990 Jun;181(3):448-50. doi: 10.1007/BF00195900.
The nature of the products of the auxin catabolism mediated by both basic and acidic isoperoxidases has been studied. While indole-3-methanol is only a minor product of the oxidation of indole-3-acetic acid catalyzed by extracellular acidic isoperoxidases, it is the only product of the oxidation of indole-3-acetic acid catalyzed by two cytosolic basic isoperoxidases (EC 1.11.1.7) from lupin (Lupinus albus L.) hypocotyls. The putative indole-3-methanol formed by these latter isoperoxidases was isolated and then characterized by mass spectrometry and (1)H-nuclear magnetic resonance spectrometry. These results are discussed with respect to the diversity and compartmentation of the catabolism of indole-3-acetic acid in plant tissues.
已研究了由碱性和酸性同功过氧化物酶介导的生长素分解产物的性质。虽然吲哚-3-甲醇只是细胞外酸性同功过氧化物酶催化的吲哚-3-乙酸氧化的次要产物,但它是两种来自羽扇豆(羽扇豆)下胚轴的胞质碱性同功过氧化物酶(EC 1.11.1.7)催化的吲哚-3-乙酸氧化的唯一产物。这些同工过氧化物酶形成的假定的吲哚-3-甲醇已被分离出来,然后通过质谱和(1)H-核磁共振光谱进行了表征。这些结果与植物组织中吲哚-3-乙酸的分解代谢的多样性和区室化有关。