玉米根中硝酸还原酶的合成与周转。
Synthesis and turnover of nitrate reductase in corn roots.
机构信息
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
出版信息
Plant Physiol. 1972 Dec;50(6):649-54. doi: 10.1104/pp.50.6.649.
Abstract
THE INDUCTION AND REINDUCTION OF NITRATE REDUCTASE IN ROOT TIP OR MATURE ROOT SECTIONS SHOW ESSENTIALLY A SIMILAR PATTERN
a lag, a period of rapid increase in enzyme activity and finally a period of relatively minor change. Both inductions are sensitive to 6-methylpurine and cycloheximide. Kinetic studies with 6-methylpurine suggest that the half-life of the messenger RNA for nitrate reductase in both sections is about 20 minutes. The rate of decay of nitrate reductase activity induced by transfer to a nitrate-free medium is slower in root tips (t(1/2) = 3 hours) than in mature root sections (t(1/2) = 2 hours). The enzyme from mature root sections is also less stable to mild heat treatments (27 C; 40 C) than the enzyme from root tip sections. The results indicate that factors regulating enzyme turnover show important changes as root cells mature and may be significant in determining steady state levels of the enzyme.
摘要
根尖端或成熟根段中硝酸盐还原酶的诱导和再诱导基本上呈现出相似的模式
一个延迟期，一个酶活性快速增加的时期，最后是一个相对变化较小的时期。这两种诱导都对 6-甲基嘌呤和环己酰亚胺敏感。用 6-甲基嘌呤进行的动力学研究表明，两种切片中硝酸盐还原酶的信使 RNA 的半衰期约为 20 分钟。转移到无硝酸盐介质中诱导的硝酸盐还原酶活性的衰减速度在根尖端（t(1/2)=3 小时）比在成熟根段（t(1/2)=2 小时）慢。与根尖端切片中的酶相比，成熟根段中的酶对温和的热处理（27°C；40°C）也不太稳定。结果表明，调节酶周转的因素随着根细胞的成熟而发生重要变化，这可能对确定酶的稳态水平具有重要意义。
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本文引用的文献
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Environmental or developmental changes cause many enzyme activities of higher plants to rise or fall.环境或发育变化会导致高等植物的许多酶活性上升或下降。
Science. 1969 Jul 25;165(3891):358-67. doi: 10.1126/science.165.3891.358.
2
Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA.高等生物中特定基因表达的调控。哺乳动物基因的表达可能受作用于信使核糖核酸翻译的阻遏物调控。
Science. 1969 Dec 19;166(3912):1474-80. doi: 10.1126/science.166.3912.1474.
3
The regulation of activity of the enzymes involved in the assimilation of nitrate by higher plants.高等植物硝酸盐同化相关酶活性的调控。
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Evidence for an Inactivating System of Nitrate Reductase in Hordeum vulgare L. during Darkness That Requires Protein Synthesis.大麦中存在一种在黑暗中使硝酸还原酶失活的系统的证据，该系统需要蛋白质合成。
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