从根瘤菌中分离出可将电子传递给大豆固氮酶的因子。
Isolation from Rhizobium for factors which transfer electrons to soybean nitrogenase.
机构信息
Department of Botany, Oregon State University, Corvallis, Oregon 97331.
出版信息
Plant Physiol. 1973 Jan;51(1):136-8. doi: 10.1104/pp.51.1.136.
Abstract
Rhizobium japonicum grown anaerobically with nitrate produced several factors capable of transferring electrons from spinach chloroplasts lacking photosystem II to soybean root nodule nitrogenase. The same organism grown aerobically produced no factors with detectable activity. It is concluded that the bacterium is potentially capable of producing these proteins in the low oxygen conditions of the leguminous root nodule.
摘要
根瘤菌在厌氧条件下利用硝酸盐产生了几种因子，这些因子能够将缺乏光系统 II 的菠菜叶绿体中的电子转移到大豆根瘤中的氮酶。同样的生物体在有氧条件下生长时，没有产生具有可检测活性的因子。因此，可以得出结论，在豆科植物根瘤的低氧条件下，这种细菌具有产生这些蛋白质的潜力。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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The plant as the genetic determinant of leghaemoglobin production in the legume root nodule.该植物作为豆科植物根瘤中豆血红蛋白产生的遗传决定因素。
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The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.固氮菌固氮过程中的电子传递系统。I. 偶氮黄素作为电子载体。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1079-86. doi: 10.1073/pnas.64.3.1079.
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The electron transport system in nitrogen fixation by Azotobacter. II. Isolation and function of a new type of ferredoxin.固氮菌固氮过程中的电子传递系统。II. 一种新型铁氧化还原蛋白的分离与功能
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1404-10. doi: 10.1073/pnas.64.4.1404.
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