来自厌氧生长的日本根瘤菌的硝酸还原酶。
Nitrate reductase from anaerobically grown Rhizobium japonicum.
作者信息
Daniel R M, Gray J
出版信息
J Gen Microbiol. 1976 Oct;96(2):247-51. doi: 10.1099/00221287-96-2-247.
PMID:993777
Abstract
The activity of nitrate reductase in Rhizobium japonicum is controlled by oxygen tension, and not by nitrate. The enzyme from R. japonicum grown anaerobically in the presence of nitrate resembles that from bacteroids in having a molecular weight of about 69000 daltons; the enzyme from aerobically grown cells ahs a molecular weight of about 170000 daltons. Both types of enzyme have similar Km values, but differ in their sensitivity to KCN.
摘要
日本根瘤菌中硝酸还原酶的活性受氧张力控制，而非硝酸盐。在硝酸盐存在下厌氧生长的日本根瘤菌中的该酶，其分子量约为69000道尔顿，与类菌体中的酶相似；需氧生长细胞中的酶分子量约为170000道尔顿。这两种酶的米氏常数相似，但对氰化钾的敏感性不同。
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