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固氮菌固氮过程中的电子传递系统。I. 偶氮黄素作为电子载体。

The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.

作者信息

Benemann J R, Yoch D C, Valentine R C, Arnon D I

出版信息

Proc Natl Acad Sci U S A. 1969 Nov;64(3):1079-86. doi: 10.1073/pnas.64.3.1079.

Abstract

A flavoprotein, named azotoflavin, was isolated from an extract of Azotobacter vinelandii cells, which linked the reducing power generated by illuminated spinach chloroplasts to the Azotobacter nitrogen-fixing enzyme complex. The photoreduction of the yellow azotoflavin by chloroplasts produced a stable, free-radical semiquinone, blue in color, with properties similar to those described by other investigators for an Azotobacter flavoprotein of unknown biological function.

摘要

从棕色固氮菌细胞提取物中分离出一种名为偶氮黄素的黄素蛋白,它将光照下的菠菜叶绿体产生的还原力与棕色固氮菌固氮酶复合物相连。叶绿体对偶氮黄素进行光还原产生了一种稳定的自由基半醌,呈蓝色,其性质与其他研究者描述的一种未知生物学功能的棕色固氮菌黄素蛋白相似。

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