分光光度法定量测定苯丙氨酸解氨酶活性可能存在的误差。
Possible errors in quantitative determination of phenylalanine ammonia-lyase activity by spectrophotometric methods.
机构信息
Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
出版信息
Plant Physiol. 1973 Feb;51(2):409-11. doi: 10.1104/pp.51.2.409.
Abstract
A possible error in spectrophotometric determination of cinnamate, the product of phenylalanine ammonia-lyase activity, using nonpurified protein extracts has been shown.Under optimal conditions for phenylalanine ammonia-lyase activity, with borate buffer and in the presence of alpha-keto acids, phenylpyruvate is produced and its enol tautomer-borate complex formed, strongly absorbs at 290 nanometers.
摘要
已显示使用非纯化蛋白提取物进行苯丙氨酸解氨酶活性产物肉桂酸的分光光度测定可能存在误差。在苯丙氨酸解氨酶活性的最佳条件下，使用硼酸缓冲液并存在α-酮酸时，会生成苯丙酮酸，其烯醇互变异构体-硼酸络合物形成，在 290 纳米处强烈吸收。
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