烟草愈伤组织培养中芽起始过程中的酪氨酸和苯丙氨酸解氨酶活性
Tyrosine and Phenylalanine Ammonia Lyase Activities during Shoot Initiation in Tobacco Callus Cultures.
作者信息
Beaudoin-Eagan L D, Thorpe T A
机构信息
Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
出版信息
Plant Physiol. 1985 Jul;78(3):438-41. doi: 10.1104/pp.78.3.438.
Abstract
Both phenylalanine ammonia lyase and tyrosine ammonia lyase were detected in tobacco (Nicotiana tabacum L. Wisconsin 38) callus. The enzymes were separated from each other by Sephadex G-200 column chromatography. Increased activity of tyrosine ammonia lyase was observed during culture of tobacco callus under shoot-forming conditions, while activity of phenylalanine ammonia lyase increased during culture under non-organ-forming conditions. Confirmation of these findings was obtained by examining the incorporation of [(14)C]tyrosine and [(14)C]phenylalanine into p-coumarate and trans-cinnamate, respectively.
摘要
在烟草（烟草品种Wisconsin 38）愈伤组织中检测到了苯丙氨酸解氨酶和酪氨酸解氨酶。通过葡聚糖凝胶G - 200柱色谱法将这两种酶彼此分离。在形成芽的条件下培养烟草愈伤组织期间，观察到酪氨酸解氨酶的活性增加，而在非器官形成条件下培养期间，苯丙氨酸解氨酶的活性增加。通过分别检测[（14）C]酪氨酸和[（14）C]苯丙氨酸掺入对香豆酸和反式肉桂酸中的情况，证实了这些发现。
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